Different MAPK signal transduction pathways play different roles in the impairment of glucose ‑stimulated insulin secretion in response to IL‑1β.

Different MAPK signal transduction pathways play different roles in the impairment of glucose‑stimulated insulin secretion in response to IL‑1β. Mol Med Rep. 2020 Jul 28;: Authors: Ou Y, Zheng Z, Niu B, Su J, Su H Abstract Mitogen‑activated protein kinase (MAPK) signal transduction pathways may be involved in the destruction of pancreatic islet β cells induced by inflammatory cytokines. The present study aimed to investigate the role of different MAPK signal transduction pathways in the interleukin‑1β (IL‑1β)‑induced inhibition of glucose‑stimulated insulin secretion (GSIS) in Min6 mouse pancreatic cells. Min6 cells were stimulated with different concentrations of glucose (0.0, 5.5, 11.1 and 22.2 mmol/l), or different concentrations of IL‑1β (0.00, 0.25 and 2.50 ng/ml) in combination with high glucose (22.2 mmol/l) and the culture supernatant was collected. The concentration of insulin was measured by enzyme‑linked immunosorbent assay and the activation of different MAPK pathways was assessed by measuring the phosphorylation levels of extracellular signal‑regulated kinase 1/2 (ERK1/2), p38 and c‑jun N‑terminal kinase (JNK) via western blotting. The production of reactive oxygen species (ROS) was determined via flow cytometry, and cell viability was detected by Cell Counting Kit‑8 assay. Reverse transcription‑quantitative PCR was used to detect the insulin 1 gene. The results revealed that glucose a...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research