Predicting the mortality of melioidosis in the heart of Borneo
Background: Melioidosis, caused by Burkholderia pseudomallei, is prevalent in rural areas of Malaysia. This soil-dwelling pathogen is inherently resistant to many first-line antibiotics and carries a high mortality rate. Hence, it poses a significant burden of disease in terms of productivity losses among farmers and blue-collar workers in resource-poor environments. There are limited reports on the factors associated with mortality from melioidosis in Borneo.
N. E. Stone et al.
Melioidosis is an infection caused by Gram-negative bacillus Burkholderia pseudomallei commonly present in the water and soil (Limmathurotsakul et al., 2013; Benoit et al., 2015). Clinically melioidosis can mimic other diseases such as cancer and tuberculosis, which in our case was suspected sarcoma.
This article is protected by copyright. All rights reserved. PMID: 33006278 [PubMed - as supplied by publisher]
We describe a general protocol for the production of ergothioneine isotopologues with programmable 2H, 15N 13C, 34S and 33S isotope labelling patterns. This enzyme-based approach makes efficient use of commercial isotope reagents and is also directly applicable for the synthesis of radio-isotopologues. PMID: 32996678 [PubMed - as supplied by publisher]
Conclusions/SignificanceOur plaque screening and genomic studies revealed evidence of impairment in plaque formation in environmental isolates ofB.pseudomallei that is associated with large genomic loss of genes important for intracellular multiplication and MNGC formation. These findings suggest that the genomic and phenotypic differences of environmental isolates may be associated with clinical infection.
Abstract Prediction models indicate that melioidosis may be common in parts of East Africa, but there are few empiric data. We evaluated the prevalence of melioidosis among patients presenting with fever to hospitals in Tanzania. Patients with fever were enrolled at two referral hospitals in Moshi, Tanzania, during 2007-2008, 2012-2014, and 2016-2019. Blood was collected from participants for aerobic culture. Bloodstream isolates were identified by conventional biochemical methods. Non-glucose-fermenting Gram-negative bacilli were further tested using a Burkholderia pseudomallei latex agglutination assay. Also, we...
Abstract A series of benzylaminoethylureido-tailed benzenesulfonamides was analyzed for their inhibition potential against bacterial carbonic anhydrases (CAs) such as VhCA α, β, and γ from Vibrio cholerae , and BpsCA β and γ-CAs from Burkholderia pseudomallei . Growing drug-resistance against antibiotics demands alternative targets and mechanisms of action. As CA is essential for the survival of bacteria, such enzymes have the potential for developing new antibiotics. Most of the compounds presented excellent inhibition potential against VhCA γ as compared to α and β, w...
Future Microbiology,Volume 15, Issue 12, Page 1109-1121, August 2020.
Abstract Melioidosis is a neglected tropical disease caused by the Gram-negative soil bacterium Burkholderia pseudomallei. Current antibiotic regimens used to treat melioidosis are prolonged and expensive, and often ineffective because of intrinsic and acquired antimicrobial resistance. Efforts to develop new treatments for melioidosis are limited by the risks associated with handling pathogenic B. pseudomallei, which restricts research to facilities with biosafety level three containment. Closely related nonpathogenic Burkholderia can be investigated under less stringent biosafety level two containment, and we hy...
Background: Splenic abscess is an uncommon condition with an estimated incidence rate of 0.05 –0.7% in autopsy studies. Causative microorganisms can be very diverse. However, in areas where melioidosis is endemic, Burkholderia pseudomallei is the most frequent cause of splenic abscess.