Click Nucleic Acid-DNA Binding Behavior: Dependence on Length, Sequence, and Ionic Strength.

Click Nucleic Acid-DNA Binding Behavior: Dependence on Length, Sequence, and Ionic Strength. Biomacromolecules. 2020 Sep 11;: Authors: Culver HR, Sinha J, Prieto TR, Calo CJ, Fairbanks BD, Bowman CN Abstract Click nucleic acids (CNAs) are a new, low-cost class of xeno nucleic acid (XNA) oligonucleotides synthesized by an efficient and scalable thiol-ene polymerization. In this work, a thorough characterization of oligo(thymine) CNA-oligo(adenine) DNA ((dA)20) hybridization was performed to guide future implementation of CNAs in applications that rely on sequence specific interactions. Microscale thermophoresis (MST) provided a convenient platform to rapidly and systematically test the effects of several factors (i.e., sequence, length, and salt concentration) on the CNA-DNA dissociation constant (Kapp). Because CNAs have limited water solubility, all studies were performed in aqueous-DMSO mixtures. CNA-DNA hybrids between oligo(thymine) CNA (average length of 16 bases) and (dA)20 DNA have good stability despite the high organic content, a favorable attribute for many emerging applications of XNAs. Specifically, the Kapp of CNA-DNA hybrids in 65 vol% DMSO with 10 mM sodium chloride (NaCl) was 0.74  0.1 μM, while the Kapp for (dT)20-(dA)20 DNA-DNA was measured to be 45  2 μM in a buffer without DMSO but at the same NaCl concentration. CNA hybridized with DNA following Watson-Crick base pairing with excellent sequence specificit...
Source: Biomacromolecules - Category: Biochemistry Authors: Tags: Biomacromolecules Source Type: research