Activation of platelet-derived growth factor receptor β in the severe fever with thrombocytopenia syndrome virus infection.

In this study, we screened the protein kinase inhibitors in inhibitory effects on the infection of Vero cells with SFTSV using InhibitorSelect™ Protein Kinase Library Series (Merck & Co., Inc., Kenilworth, NJ, USA). Several types of inhibitors targeted to platelet-derived growth factor receptor β (PDGFRβ) inhibited the infection of Vero, Huh7, and NIH3T3 cells with SFTSV in a dose-dependent manner within the noncytotoxic range. In addition, these protein kinase inhibitors also inhibited the infection of the target cells with SFTSV glycoprotein (SFTSV-GP) pseudotyped virus (SFTSVpv). Activation of PDGFRβ phosphorylation was detected in SFTSV-treated cells. The infectivities of SFTSVpv were specifically decreased not only in NIH3T3 cells treated with siRNA for PDGFRβ but also in NIH3T3 cells treated with anti-PDGFRβ neutralizing antibody in a dose-dependent manner. SFTSV growth and entry of SFTSVpv were also inhibited by Akt inhibitors. Activation of Akt phosphorylation was also detected in SFTSV-treated cells. These data indicate that PDGFRβ is one of the important host factors in the entry steps of SFTSV.
PMID: 32882323 [PubMed - as supplied by publisher]

Source: Antiviral Research - August 30, 2020 Category: Virology Authors: Tani H, Kimura M, Yamada H, Fujii H, Taniguchi S, Shimojima M, Fukushi S, Morikawa S, Saijo M Tags: Antiviral Res Source Type: research