High-resolution 3D bioprinting of photo-crosslinkable recombinant collagen to serve tissue engineering applications.

High-resolution 3D bioprinting of photo-crosslinkable recombinant collagen to serve tissue engineering applications. Biomacromolecules. 2020 Aug 25;: Authors: Tytgat L, Dobos A, Markovic M, Van Damme L, Van Hoorick J, Bray F, Thienpont H, Ottevaere H, Dubruel P, Ovsianikov A, Van Vlierberghe S Abstract Various biopolymers including gelatin, have already been applied to serve a plethora of tissue engineering purposes. However, substantial concerns have arisen related to the safety and the reproducibility of these materials due to their animal origin and the risk associated with pathogen transmission as well as batch-to-batch variations. Therefore, researchers have been focussing their attention towards recombinant materials that can be produced in a laboratory with full reproducibility and can be designed according to specific needs (e.g. by introducing additional RGD sequences). In the present study, a recombinant protein based on collagen type I (RCPhC1) was functionalized with photo-crosslinkable methacrylamide (RCPhC1-MA), norbornene (RCPhC1-NB) or thiol (RCPhC1-SH) functionalities to enable high resolution 3D-printing via two-photon polymerization (2PP). The results indicated a clear difference in 2PP processing capabilities between the chain-growth polymerized RCPhC1-MA and the step-growth polymerized RCPhC1-NB/SH. More specifically, reduced swelling-related deformations resulting in a superior CAD-CAM mimicry were obtained for ...
Source: Biomacromolecules - Category: Biochemistry Authors: Tags: Biomacromolecules Source Type: research