Method of treating central nervous system disease

 Blood-Brain Barrier (BBB) Permeability AssayYes, These are troubled times. With our current focus on eradicating COVID-19, we forget that many of the world ' s most insidious and costly diseases have limited treatments and no cures.That is why Neuromics continues to develop its human based 3-D Cell-Based Assays. At the center if our offerings is ourBBB Model.Here ' s a recent patent application that includes a permeability assay using our model-Sookhee Bang, Jeong Kuen Song, Seung-wook Shin, Kwan Hee Lee, and Ho June Lee. (2020).Method of Treating Central Nervous System Disease. United States Patent Application 20200230218BBB Permeability Assay of AL04The in vitro human BBB model (Neuromics, USA) was established using co-cultures of primary Human brain endothelial cells (HBEC), Human brain pericytes (HBPC), and Human brain astrocytes (HBAC). In vitro human BBB model kit has two sides (luminal, blood/abluminal, brain) with 12 transwell inserts (polyester membrane, 0.4 um pore, diameter 12 mm, insert growth area: 1.12 cm2). HBPC were grown on the bottom side of the inserts, HBEC were monolayered on the upper side of the inserts, and HBAC were grown on the bottom of the 12-well culture plate (Neuromics, USA). In vitro BBB model was activated according to the manufacturer ' s instructions for 4 days. Briefly, the medium from both luminal and abluminal (lower, brain) sides of the transwell insert was changed every other day. Before the transport experiment, the abluminal sid...
Source: Neuromics - Category: Neuroscience Tags: 3-D Assays. 3-D imaging 3-D Blood-Brain Barrier Model 3-D Cell Culturing Blood Brain Barrier Blood Brain Barrier Models –brain barrier penetration Source Type: news