Application of Proteomics in Sarcoidosis.

Application of Proteomics in Sarcoidosis. Am J Respir Cell Mol Biol. 2020 Aug 17;: Authors: Guerrero CR, Maier LA, Griffin TJ, Higgins L, Najt CP, Perlman DM, Bhargava M Abstract Sarcoidosis is a multisystem disease with heterogeneity in manifestations and outcomes. System-level studies leveraging "omics" technologies are expected to define mechanisms contributing to sarcoidosis heterogeneous manifestations and course. With improvements in mass spectrometry (MS) and bioinformatics, it is possible to study protein abundance for a large number of proteins simultaneously. Contemporary fast-scanning MS enables acquisition of spectral data for deep coverage of the proteins with data-dependent acquisition-MS or data-independent acquisition-MS modes. Studies leveraging MS-based proteomics in sarcoidosis have characterized bronchoalveolar lavage fluid (BALF), alveolar macrophages, plasma, and exosomes. These studies identified several differentially expressed proteins, including protocadherin-2 precursor, annexin A2, pulmonary surfactant A2, complement factors C3, vitamin-D binding protein, cystatin B, amyloid P comparing sarcoidosis to controls. Other studies identified ceruloplasmin, complement factor B, C3 and 1 and others with differential abundance in sarcoidosis compared to other interstitial lung diseases. Using quantitative proteomics, most recent studies found differences in PI3K/Akt/ mTOR, MAP kinase, pluripotency-associated transc...
Source: American Journal of Respiratory Cell and Molecular Biology - Category: Molecular Biology Authors: Tags: Am J Respir Cell Mol Biol Source Type: research