Molecular Markers for Detecting Schistosoma Species by Loop-Mediated Isothermal Amplification.

Molecular Markers for Detecting Schistosoma Species by Loop-Mediated Isothermal Amplification. Dis Markers. 2020;2020:8042705 Authors: Fernández-Soto P, Avendaño C, Sala-Vizcaíno A, Crego-Vicente B, Febrer-Sendra B, García-Bernalt Diego J, Oleaga A, López-Abán J, Vicente B, Patarroyo MA, Muro A Abstract Schistosomiasis is considered a neglected parasitic disease. Around 280,000 people die from it annually, and more than 779 million people are at risk of getting infected. The schistosome species which infect human beings are Schistosoma mansoni, Schistosoma haematobium, Schistosoma intercalatum, Schistosoma japonicum, Schistosoma guineensis, and Schistosoma mekongi. This disease is also of veterinary significance; the most important species being Schistosoma bovis since it causes the disease in around 160 million livestock in Africa and Asia. This work was aimed at designing and developing a genus-specific loop-mediated isothermal amplification (LAMP) method for detecting the most important schistosome species affecting humans and for the species-specific detection of S. bovis. Bioinformatics tools were used for primer design, and the LAMP method was standardised for detecting the ITS-1 region from S. intercalatum, S. haematobium, S. mansoni, S. japonicum, and S. bovis DNA (generic test) and the NADH 1 gene for specifically detecting S. bovis (at different DNA concentrations). Detection limits achieved were 1 pg DNA for S. man...
Source: Disease Markers - Category: Laboratory Medicine Tags: Dis Markers Source Type: research