Simultaneous quantitative assessment of two distinct cell lineages with a nuclear-localized dual genetic reporter

Genetic lineage tracing has been widely used for studying in vivo cell fate plasticity during embryogenesis, tissue homeostasis, and disease development. Recent applications with multiple site-specific recombinases have been used in complex and sophisticated genetic fate mapping studies. However, the previous multicolor reporters for dual recombinases had limitations of precise in situ quantification of cell number, which is mainly due to the intermingling of cells in condensed tissues. Here, we generated a dual recombinase-mediated nuclear-localized GFP and tdTomato reporter line, which enables clear, simultaneous quantification of two distinct cell lineages in vivo.

Source: Journal of Molecular and Cellular Cardiology - July 11, 2020 Category: Cytology Authors: Muxue Tang, Kuo Liu, Hengwei Jin, Yan Li, Shaohua Zhang, Xiuxiu Liu, Ximeng Han, Maoying Han, Zhenqian Zhang, Bin Zhou Source Type: research