SAND FLY TYPING: A SIMPLE AND MORPHOLOGICALLY-SUPPORTED METHOD BASED ON POLYMORPHISM OF 18S rRNA GENE IN A LEISHMANIASIS ENDEMIC AREA OF ARGENTINA.

SAND FLY TYPING: A SIMPLE AND MORPHOLOGICALLY-SUPPORTED METHOD BASED ON POLYMORPHISM OF 18S rRNA GENE IN A LEISHMANIASIS ENDEMIC AREA OF ARGENTINA. Acta Trop. 2020 Jun 26;:105609 Authors: Almazán MC, Copa GN, Lauthier JJ, Gil JF, Quiroga IL, Hoyos CL, Fernández MED, Nasser JR, Korenaga M, Marco JD, Barroso PA Abstract Leishmaniases are vector-borne diseases that in the Americas are distributed from southern United States to northern Argentina. The vectors for this disease are small dipterans known as sand flies that are usually identified morphologically by observing structures with taxonomic value; but it is time-consuming, laborious, and requires entomological expertise. Then, this work was aimed at identifying sand flies with molecular techniques, using the morphological identification as a reference technique, in an endemic area of American Tegumentary Leishmaniasis (ATL) located in northern Argentina. For this, sand flies were caught at two patches of vegetation adjacent to rural areas in Orán department, Salta Province. Females were dissected with sterile needles; the head and last abdominal segments were analyzed for morphological identification. The remaining thorax and abdominal segments were used to extract DNA, which was amplified by PCR of the small subunit (SSU), 18S rRNA gene. PCR products were digested with CviQI and DdeI enzymes to identify sand fly species by Restriction Fragment Length Polymorphism (RFLP) analysi...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research