Isolation and molecular identification of Aspergillus flavus and the study of its potential for malathion biodegradation in water

This study was carried out for isolation and molecular identification of microorganisms from water for malathion biodegradation in aquatic system. PCR analysis was used for identification of the isolated fungus. The growth kinetics ofA. flavus in the presence of malathion under different environmental factors (pH, temperature and malathion concentration) were evaluated. Furthermore, the degradation kinetics of malathion byA. flavus in aqueous media under different environmental factors was evaluated. The isolated microorganism was identified asA. flavus with respect to it relation to the data from the gene bank and the lowest nucleotide diversity value between the tested isolate andA. flavus. The identified isolate grew successfully in a media supplemented with malathion much faster than without it. Hundred percent of malathion initial concentration was degraded within 36  days of incubation withA. flavus. The temperature of 30  °C, pH value of 7 and malathion initial concentration of 5 mg/l were the optimum conditions ofA. flavus for growth and degradation of malathion. Bioremediation of malathion residues in water usingA. flavus isolate are promising and considered the first report.
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research