The need to freeze ‐ dehydration during specimen preparation for electron microscopy collapses the endothelial glycocalyx regardless of fixation method

ConclusionsBoth chemical and cryogenic fixation followed by dehydration lead to substantial collapse of the glycocalyx. Cryogenic fixation without freeze substitution could be a way forward although transmission soft x ‐ray tomography based solely on amplitude contrast seems unsuitable.

Source: Microcirculation - June 15, 2020 Category: Research Authors: Casper Hempel, Sergey Kapishnikov, Ana Joaquina Perez ‐Berna, Stephan Werner, Peter Guttmann, Eva Pereiro, Klaus Qvortrup, Thomas Lars Andresen Tags: ORIGINAL RESEARCH Source Type: research