Expression analysis of defense-related genes in cucumber (Cucumis sativus L.) against Phytophthora melonis.

Expression analysis of defense-related genes in cucumber (Cucumis sativus L.) against Phytophthora melonis. Mol Biol Rep. 2020 Jun 12;: Authors: Hashemi L, Golparvar AR, Nasr-Esfahani M, Golabadi M Abstract Phytophthora melonis is one of the most destructive cucumber disease, causing severe economic losses in the globe. Despite intense research efforts made in the past years, no permanent cure currently exists for this disease. With the aim to understand the molecular mechanisms of defense against P. melonis, root collars and leaves of four cucumber genotypes consisting of resistant Ramezz; moderately resistant Baby, and very susceptible Mini 6-23 and Extrem, were monitored for quantitative gene expression analysis of the five antifungal and/or anti-oomycete genes (CsWRKY20, CsLecRK6.1, PR3, PR1-1a and LOX1), at three points after inoculation with P. melonis. The gene expression analysis indicated, P. melonis strongly enhanced the expression of these genes after inoculation, in both the leaves and root collars. Further, not only the transcript levels of these genes were significantly higher in resistant and moderately resistance genotypes, but also the time point of the highest relative expression ratio for the five genes was different in the four cucumber genotypes. CsWRKY20 and PR3 showed the maximum expression in Ramezz at 48 h post inoculation (hpi) while CsLecRK6.1, and LOX1 showed the highest expression at 72 hpi. In addition,...
Source: Molecular Biology Reports - Category: Molecular Biology Authors: Tags: Mol Biol Rep Source Type: research