Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo.

Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo. BMB Rep. 2020 Jun 01;: Authors: Choo YW, Jeong J, Jung K Abstract Currently, most biological researches rely on conventional experimental techniques that only allow static analyses at certain time points in vitro or ex vivo. However, if one can visualize cellular dynamics in living organisms, it would provide a unique opportunity to study key biological phenomena in vivo. Intravital microscopy (IVM) encompasses diverse optical systems for direct visualization of objects including biological structures and individual cells in live animals. With the current development of devices and techniques, IVM addresses important questions in various fields of biological and biomedical sciences. In this mini-review, we provide a general introduction of IVM and examples of recent applications in the field of immunology, oncology, and vascular biology. We also introduce an advanced type of IVM, dubbed real-time IVM, equipped with video-rate resonant scanning. Since the real-time IVM can render cellular dynamics with high temporal resolution in vivo, it allows visualization and analysis of rapid biological processes. PMID: 32475382 [PubMed - as supplied by publisher]
Source: BMB Reports - Category: Biochemistry Authors: Tags: BMB Rep Source Type: research