Ion transport and regulation in a synaptic vesicle glutamate transporter

We report the cryo–electron microscopy structure of rat VGLUT2 at 3.8-angstrom resolution and propose structure-based mechanisms for substrate recognition and allosteric activation by low pH and chloride. A potential permeation pathway for chloride intersects with the glutamate binding site. These results demonstrate how the activity of VGLUTs can be coordinated with large shifts in proton and chloride concentrations during the synaptic vesicle cycle to ensure normal synaptic transmission.

Source: ScienceNOW - May 20, 2020 Category: Science Authors: Li, F., Eriksen, J., Finer-Moore, J., Chang, R., Nguyen, P., Bowen, A., Myasnikov, A., Yu, Z., Bulkley, D., Cheng, Y., Edwards, R. H., Stroud, R. M. Tags: Biochemistry reports Source Type: news