Surfactant-secreted phospholipase A2 interaction and respiratory outcome in preterm neonates.

Surfactant-secreted phospholipase A2 interaction and respiratory outcome in preterm neonates. Am J Physiol Lung Cell Mol Physiol. 2020 May 13;: Authors: De Luca D, Shankar-Aguilera S, Autilio C, Raschetti R, Vedovelli L, Fitting C, Payré C, Jeammet L, Pérez-Gil J, Cogo PE, Carnielli VP, Lambeau G, Touqui L Abstract Secreted phospholipase A2 hydrolyses surfactant phospholipids and is crucial for the inflammatory cascade; preterm neonates are treated with exogenous surfactant but the interaction between surfactant and phospholipase is unknown. We hypothesize that this interplay is complex and the enzyme plays a relevant role in neonates needing surfactant replacement. We aimed to: 1) identify phospholipases A2 isoforms expressed in preterm lung; 2) study the enzyme role on surfactant re-treatment and function and the effect of exogenous surfactant on the enzyme system; 3) verify if phospholipase A2 is linked to respiratory outcomes. In bronchoalveolar lavages of preterm neonates, we measured enzyme activity (alone or with inhibitors), enzyme subtypes, surfactant protein-A and inflammatory mediators. Surfactant function and phospholipid profile were also tested. Urea ratio was used to obtain epithelial lining fluid concentrations. Follow-up data were prospectively collected. Subtype-IIA is the main phospholipase isoform in preterm lung, although subtype-IB may be significantly expressed. Neonates needing surfactant retreatment have hi...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - Category: Cytology Authors: Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research