New effects of caffeine on CRH-induced stress along the intrafollicular classical HPA-axis (CRH-R1/2, IP3 -R, ACTH, MC-R2) and the neurogenic non-HPA-axis (substance P, p75NTR and TrkA) in ex vivo human male androgenetic scalp hair follicles.

New effects of caffeine on CRH-induced stress along the intrafollicular classical HPA-axis (CRH-R1/2, IP3 -R, ACTH, MC-R2) and the neurogenic non-HPA-axis (substance P, p75NTR and TrkA) in ex vivo human male androgenetic scalp hair follicles. Br J Dermatol. 2020 Apr 09;: Authors: Fischer TW, Bergmann A, Kruse N, Kleszczynski K, Skobowiat C, Slominski AT, Paus R Abstract Human hair is highly responsive to stress, and human scalp hair follicles (HFs) contain a peripheral neuro-endocrine equivalent of the systemic hypothalamic-pituitary-adrenal (HPA) stress axis. Androgenetic alopecia (AGA) is supposed to be aggravated by stress. We used the HPA axis triggering corticotropin-releasing hormone (CRH) to induce a stress response in human ex vivo male AGA HFs. Furthermore, since caffeine is known to reverse testosterone-mediated hair growth inhibition in the same hair organ culture model, we investigated whether caffeine would antagonize CRH-mediated stress in these HFs. HFs from balding vertex area scalp biopsies of AGA-affected men were incubated with CRH (10-7 M) and with/without caffeine (0.001%, 0.005%). Compared to controls, CRH significantly enhanced the expression of catagen-inducing TGF-β2 (p<0.001), CRH receptors 1/2 (p<0.01), ACTH (p<0.001) and melanocortin-receptor-2 (MC-R2) (p<0.001), and additional stress-associated parameters, substance P and p75 neurotrophin receptor. CRH inhibited matrix keratinocyte proliferat...
Source: The British Journal of Dermatology - Category: Dermatology Authors: Tags: Br J Dermatol Source Type: research