Biotransformation of glycosylated saponins in balloon flower root extract into 3-O- β-D-glucopyranosyl platycosides by deglycosylation of pectinase from Aspergillus aculeatus.

Biotransformation of glycosylated saponins in balloon flower root extract into 3-O-β-D-glucopyranosyl platycosides by deglycosylation of pectinase from Aspergillus aculeatus. J Microbiol Biotechnol. 2020 Mar 13;: Authors: Ju JH, Kang SH, Kim TH, Shin KC, Oh DK Abstract Platycodon grandiflorum root (Platycodi radix) saponins, platycosides, have been used as health supplements and food items for the treatment of respiratory disorders and pulmonary diseases. Deglycosylated saponins have been known to exert stronger biological effects than their glycosylated forms. In the present study, glycosylated platycosides in Platycodi radix extract were biotransformed into deglycosylated 3-O-β-D-glucopyranosyl platycosides, including 3-O-β-D-glucopyranosyl platycodigenin, 3-O-β-D-glucopyranosyl polygalacic acid, and 3-O-β-D-glucopyranosyl platyconic acid, by pectinase from Aspergillus aculeatus. This is the first report on the quantitative enzymatic production of 3-O-β-D-glucopyranosyl platycosides. The chemical structures of 3-O-β-D-glucopyranosyl platycosides were identified with LC/MS. The biotransformation pathways of three-type platycosides in Platycodi radix into 3-O-β-D-glucopyranosyl platycosides were established. PMID: 32238760 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research