Imaging Mass Cytometry to Assess the Pathology of Chronic Lung Allograft Dysfunction

The long-term survival after lung transplantation remains limited, mostly because of Chronic Lung Allograft Dysfunction (CLAD), which has two different phenotypes: bronchiolitis obliterans syndrome (BOS) and restrictive allograft syndrome (RAS). Our tools for assessing lung allograft cells are limited by the autofluorescence of the lung tissue. We aimed to assess CLAD lung allografts using imaging mass cytometry (IMC), a mass spectroscopic technology that allows high-resolution, multi-dimensional characterization of lung cell surface and intracellular proteins at a single cell level, using heavy metal-tagged antibodies.

Source: The Journal of Heart and Lung Transplantation - March 31, 2020 Category: Transplant Surgery Authors: B. Renaud-Picard, C. Qing, O. Ornatsky, S. Moshkelgosha, D. Hwang, S. Juvet, T. Martinu Tags: (419) Source Type: research