Candida albicans Beta-Glucan Induce Anti- Cancer Activity of Mesenchymal Stem Cells against Lung Cancer Cell Line: An In-Vitro Experimental Study.

Candida albicans Beta-Glucan Induce Anti- Cancer Activity of Mesenchymal Stem Cells against Lung Cancer Cell Line: An In-Vitro Experimental Study. Asian Pac J Cancer Prev. 2020 Mar 01;21(3):837-843 Authors: Peymaeei F, Sadeghi F, Safari E, Khorrami S, Falahati M, Roudbar Mohammadi S, Roudbary M Abstract OBJECTIVE: β-glucan, glucopyranosyl polymers of fungi cell wall, represent an immune stimulating effects with potential anti-cancer activity. Mesenchymal stem cells (MSC) have immunomodulating properties in cancer microenvironment. The aim of this study was to investigate the anti-cancer effect of Candida albicans (C. albicans) beta-glucan on MSCs supernatant for apoptosis assay of lung cancer cells in vitro. METHODS: Beta-glucan was extracted from cell wall of C.albicans. MSC isolated from adipose tissue of patients and confirmed using specific surface markers expression which examined by flow cytometry. MSCs treated with various concentrations of β-glucans for 48 hours. Cytotoxic effect of β-glucans was evaluated using MTT assay. MSC and lung cancer line cocultured and treated with β-glucans and apoptosis assay was done by flow cytometry. RESULTS: Cytotoxicity findings showed a significant decrease in MSC viability during 48h, however it was dose-dependent (P
Source: Asian Pacific Journal of Cancer Prevention - Category: Cancer & Oncology Tags: Asian Pac J Cancer Prev Source Type: research

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This study retrospectively analyzed the clinical data of 411 patients with lung cancer who were hospitalized in the First Affiliated Hospital of Zhejiang University Medical College from January 2017 to December 2018, and counted the incidence of nosocomial lung infection, pathogens, drug resistance and influencing factors. Results There were 184 cases of nosocomial pulmonary infection in 411 lung cancer patients during chemotherapy, the infection rate was 44.77%. The isolated pathogens included Gram-negative bacteria, Gram-positive bacteria, viruses, fungi and tuberculosis, among which Gram-negative bacteria accounted for ...
Source: Chinese Journal of Lung Cancer - Category: Cancer & Oncology Source Type: research
Santosh K. Ghosh1*, Thomas S. McCormick1,2 and Aaron Weinberg1* 1Biological Sciences, School of Dental Medicine, Case Western Reserve University, Cleveland, OH, United States 2Dermatology, School of Medicine, Case Western Reserve University, Cleveland, OH, United States Human beta-defensins (hBDs, −1, 2, 3) are a family of epithelial cell derived antimicrobial peptides (AMPs) that protect mucosal membranes from microbial challenges. In addition to their antimicrobial activities, they possess other functions; e.g., cell activation, proliferation, regulation of cytokine/chemokine production, migration, diffe...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
In this study, the three natural compounds 6-methoxymellein (3), angelicoin B (4) and ellagic acid as well as nine novel 3,4-dihydroisocoumarins (Figure 1) were analyzed regarding their cytotoxicity in cancer cells and inhibition of the endogenously expressed human ABC transporters P-gp, BCRP, and MRP1 and of the yeast transporter Pdr5. For further insights into the mechanism of action, Pdr5 ATPase and substrate transport assays were performed. These results were complemented with molecular docking studies that indicate that differences in the inhibitory power of the investigated 3,4-dihydroisocoumarins with respect to P-g...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
In this study they also showed PTX3 localized in NETs formed after neutrophil activation (5). Proteomics analysis revealed that PTX3 forms complexes with two anti-microbial proteins [azurocidin (AZU1) and myeloperoxidase (MPO)] associated to NETs (30). More recently, PTX3 localization in NETs has been confirmed, and the colocalization with AZU1 and MPO has been defined more accurately (31). Further investigation will be needed to understand the involvement of PTX3 interaction with AZU1 and MPO in their antibacterial role during NET formation. Regulation of Complement Activation PTX3 interaction with microorganisms is not...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Publication date: June 2019Source: Phytochemistry Letters, Volume 31Author(s): Eleftheria Michalakea, Konstantia Graikou, Nektarios Aligiannis, George Panoutsopoulos, Eleftherios Kalpoutzakis, Christos Roussakis, Ioanna ChinouAbstractIn the framework of phytochemical studies on Greek endemic Inula species, Inula candida subsp. candida and Inula candida subsp. decalvans have been analyzed. The aerial parts were extracted with increasing polarity solvents and sixteen secondary metabolites from I. candida subsp. candida and eight from I. candida subsp. decalvans were isolated. From I. candida subsp. decalvans a sesquiterpene ...
Source: Phytochemistry Letters - Category: Chemistry Source Type: research
Abstract New Pd(II) and Pt(IV) triazine complexes [Pt3(L 1 )2(Cl)9(H2O)3].3Cl.3H2O (1), [Pt3(L 2 )2(Cl)9(H2O)3].3Cl (2), [Pt3(L 3 )2(Cl)9(H2O)3].3Cl (3), [Pt2(L 4 )2(Cl)6(H2O)2] .2Cl.4H2O (4), [Pd3(L 1 )2(H2O)6] .3Cl2 (5), [Pd3(L 2 )2(H2O)6].3Cl2 (6), [Pd3(L 3 )2(H2O)6].3Cl2 (7), and [Pd2(L 4 )2(H2O)4].2Cl2 (8) were synthesized and well characterized using elemental analyses, molar conductance, IR, UV-Vis, magnetic susceptibility, 1H, 13C-NMR spectra, and thermal analyses. These analyses deduced that the L 1 , L 2 , and L 3 ligands act as tridentate forming octahedral geometry with Pt(IV) metal ions and square pla...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
Abstract The chemical composition and biological activities of the essential oil (EO) from the rhizomes of Zingiber striolatum Diels were reported for the first time. Forty-five compounds were identified, and represented 95.7% of the total composition of the EO. The predominant components of the EO were β-phellandrene (24.0%), sabinene (17.3%), β-pinene (11.4%), geranyl linalool (8.6%), terpinen-4-ol (8.3%), α-pinene (5.6%) and crypton (4.5%). The EO revealed a weak DPPH and ABTS radical-scavenging activity. The EO exhibited significant antimicrobial activity with the inhibition zones (12.86-24.62&...
Source: Natural Product Research - Category: Biochemistry Authors: Tags: Nat Prod Res Source Type: research
Infectious diseases are one of the leading causes of death (35-70%) in lung cancer patients. We assessed the relationship between lung cancer and infection of the bronchial tree by broncho lavage using fibrobronchoscopy (FBS); among patients with lung cancer and pulmonary infection (A: n=29), patients with lung cancer (B n=85) and patients with pulmonary infections only (C: n=16). 183 patients undergoing diagnostic FBS with sampling for microbiological examination by bronchial lavage and for cytology/histology by biopsy, brushing or needle aspiration was enrolled. Pathogenic microorganisms were isolated in 25.4% of neoplas...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Respiratory Infections Source Type: research
Abstract The basic goal of this study was to synthesize zinc oxide nanoparticles using the Chelidonium majus extract and asses their cytotoxic and antimicrobial properties. The synthesized ZnO NPs were characterized by UV-Vis, Scanning Electron Microscopy (SEM) with EDS profile, Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM). The aforementioned methods confirmed that the size of synthesized ZnO nanoparticles was at the range of 10 nm. The antimicrobial activity of ZnO nanoparticles synthesized using the Ch. maju...
Source: Biomedical Microdevices - Category: Biomedical Engineering Authors: Tags: Biomed Microdevices Source Type: research
In this study, a novel peptide belonging to the phylloseptin family of antimicrobial peptides was isolated from the skin secretion of the Phyllomedusa camba, namely phylloseptin-PC (PSN-PC). The biosynthetic precursor was obtained by molecular cloning and the mature peptide sequence was confirmed through tandem mass spectrometry (MS/MS) fragmentation sequencing in the skin secretion. The synthetic replicate exhibited a broad spectrum antimicrobial activity against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans at concentrations of 2, 2, 8, 32 a...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
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