Magnetic Resonance Imaging of Single Cells.

Magnetic Resonance Imaging of Single Cells. Methods Mol Biol. 2020;2126:95-106 Authors: Kalubowilage M, Bossmann SH Abstract This chapter discusses a methodology for simultaneously imaging stem cells and endothelial cells within polysaccharide-based scaffolds for tissue engineering. These scaffolds were then implanted into nude mice. Human mesenchymal stem cells (HMSCs) were labeled with the T1-marker Gd(III)-DOTAGA-functionalized polysiloxane nanoparticles (GdNPs), whereas endothelial umbilical vein cells (HUVECs) were labeled with citrate-stabilized maghemite nanoparticles (IONPs), which predominantly shorten the T2-relaxation times of the water molecules in scaffolds and tissue. Dual cell detection was achieved by performing T1- and T2-weighted MRI in both tissue scaffolds and in vivo. PMID: 32112382 [PubMed - as supplied by publisher]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research