Isolation of Thermostable Lignocellulosic Bacteria From Chicken Manure Compost and a M42 Family Endocellulase Cloning From Geobacillus thermodenitrificans Y7

The composting ecosystem provides a potential resource for finding new microorganisms with the capability for cellulose degradation. In the present study, Congo red method was used for the isolating of thermostable lignocellulose-degrading bacteria from chicken manure compost. A thermophilic strain named as Geobacillus thermodenitrificans Y7 with acid-resident property was successfully isolated and employed to degrade raw switchgrass at 60°C for 5 days, which resulted in the final degradation rates of cellulose, xylan, and acid-insoluble lignin as 18.64, 12.96, and 17.21%, respectively. In addition, GC-MS analysis about aromatic degradation affirm the degradation of lignin by G. thermodenitrificans Y7. Moreover, an endocellulase gene belong to M42 family was successfully cloned from G. thermodenitrificans Y7 and expressed in Escherichia coli BL21. Recombinant enzyme Cel-9 was purified by Ni-NTA column based the His-tag, and the molecular weight determined as 40.4 kDa by SDA–PAGE. The characterization of the enzyme Cel-9 indicated that the maximum enzyme activity was realized at 50°C and pH 8.6 and, Mn2+ could greatly improve the CMCase enzyme activity of Cel-9 at 10 mM, which was followed by Fe2+ and Co2+. Besides, it also found that the β-1,3-1,4, β-1,3, β-1,4, and β-1,6 glucan linkages all could be hydrolyzed by enzyme Cel-9. Finally, during the application of enzyme Cel-9 to switchgrass, the saccharification rates achieved to 1.81 ±...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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Publication date: Available online 27 March 2020Source: Biochimica et Biophysica Acta (BBA) - General SubjectsAuthor(s): Angela Di Somma, Concetta Avitabile, Arianna Cirillo, Antonio Moretta, Antonello Merlino, Luigi Paduano, Angela Duilio, Alessandra Romanelli
Source: Biochimica et Biophysica Acta (BBA) General Subjects - Category: Biochemistry Source Type: research
Conclusion: This study has a total complication rate of 16%, approximately 10% of those are severe. The most common complication to PCNL was infection (60%), followed by bleeding (5.4%), reoperation (1.6%) and pain (0.5%). The high prevalence of E. faecalis might need to be considered, however the results should be validated in a larger cohort, possibly with a higher rate of antibiotic resistance, before a change of guidelines regarding prophylactic antibiotics could be proposed. PMID: 32208808 [PubMed - as supplied by publisher]
Source: Scandinavian Journal of Urology - Category: Urology & Nephrology Tags: Scand J Urol Source Type: research
Analyst, 2020, Advance Article DOI: 10.1039/C9AN02572C, PaperBin Yang, Yiling Fan, Yang Li, Jun Yan, Xueen Fang, Jilie Kong An integrated microfluidic system based on circular fluorescent probe-mediated isothermal nucleic acid amplification for identification of five diarrheagenicEscherichia coli strains has been developed. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Analyst latest articles - Category: Chemistry Authors: Source Type: research
The objectives of this study were the green synthesis of bismuth oxide nanoparticles using aqueous extract with a good potential for UV blocking and antibacterial activity. Based on the obtained results, Bi2O3 NPs can have a good candidate for different applications.
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research
Conclusion: These observations suggest that early onset colorectal cancer and AML may be related diseases.
Source: American Journal of Clinical Oncology - Category: Cancer & Oncology Tags: Original Articles: Gastrointestinal Source Type: research
In this study, we analyzed the epidemiological and genetic features of NoV outbreaks from July 2014 to June 2018 in Huzhou, China. A total of 450 stool samples collected from 51 AGE outbreaks were tested for NoVs by real-time RT PCR. Partial polymerase and capsid sequences of NoV-positive samples were amplified and sequenced for phylogenetic analysis. NoVs were found to be responsible of 84.3% of AGE outbreaks in Huzhou over the past 5 years. Most NoV outbreaks were reported in the cool months (November-March) and occurred in primary schools and kindergartens. Changes in the diversity of genotypes and the distribution of p...
Source: Archives of Virology - Category: Virology Source Type: research
Bacterial persisters are rare phenotypic variants that are temporarily tolerant to high concentrations of antibiotics. We have previously discovered that stationary-phase-cell subpopulations exhibiting high redox activities were less capable of producing proteins and resuming growth upon their dilution into fresh media. The redox activities of these cells were maintained by endogenous protein and RNA degradation, resulting in self-inflicted damage that transiently repressed the cellular functions targeted by antibiotics. Here, we showed that pretreatment of stationary-phase cells with an ATP synthase inhibitor, chlorpromaz...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, the stress response of Campylobacter coli and Campylobacter lari to elevated temperatures (46°C) was investigated by survival assays and whole transcriptome analysis. None of the strains survived at 46°C for more than 8 h and approximately 20% of the genes of C. coli RM2228 and C. lari RM2100 were differentially expressed. The transcriptomic profiles showed enhanced gene expression of several chaperones like dnaK, groES, groEL, and clpB in both strains, indicating a general involvement in the heat stress response within the Campylobacter species. However, the pronounced differences in the expression ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
AbstractThe aim of the present study was to evaluate the effect of a humic acid (HA) ‐supplemented diet on productive performance and some physiological parameters of growing rabbits. A total of 80 weaned V‐line rabbits at an age of 4 weeks were randomly divided into four groups. Rabbits of groups 2, 3 and 4 were fed diet containing 35 (HA35), 70 (HA70) and 105 (HA105) mg Humic acid/kg diet while the first group served as control (HA0). Body weight gain was positively affected by HA treatment. HA105 rabbits had the improved feed conversion value. HA treatments had significantly reduced plasma cholesterol concentra...
Source: Journal of Animal Physiology and Animal Nutrition - Category: Zoology Authors: Tags: ORIGINAL ARTICLE Source Type: research
Publication date: Available online 25 March 2020Source: Research in Veterinary ScienceAuthor(s): Mei Xue, Muhammad Akmal Raheem, Yi Gu, Huiqi Lu, Xiangjun Song, Jian Tu, Ting Xue, Kezong Qi
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
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