Neuroprotective effects of miR-532-5p against ischemic stroke

In this study, we established anin vivo middle cerebral artery occlusion (MCAO) model in mice. The expression level of miR-532-5p, neurological score, infarct area, neuronal apoptosis, and phosphoinositide 3-kinase (PI3K)/Akt signaling pathway-related molecules were examined. Low miR-532-5p levels and high phosphatase and tensin homolog deleted on chromosome 10 (PTEN) levels were detected in the mouse MCAO model. MiR-532-5p overexpression improved neurological dysfunction, reduced the infarct area, attenuated neuronal injury and apoptosis, and promoted the activation of the PI3K/Akt signaling pathway in MCAO mice. In vitro, we treated mouse neuroblastoma cells (N2a) with oxygen-glucose deprivation and reperfusion (OGD/R). The expression level of miR-532-5p, cell viability, cell apoptosis, and the PI3K/Akt signaling pathway-related molecules were detected. Consistent with thein vivo tests, the miR-532-5p level was decreased and the PTEN level was increased in OGD-treated N2a cellsin vitro. The miR-532-5p mimic increased cell viability, decreased cell apoptosis, and activated the PI3K/Akt signaling pathway. Furthermore, PTEN was verified as a target gene of miR-532-5p by luciferase reporter assay. PTEN overexpression attenuated the protective effect of miR-532-5p in OGD-treated N2a cells. In summary, these findings reveal that miR-532-5p protects against ischemic stroke by inhibiting PTEN and activating the PI3K/Akt signaling pathway and may serve as a novel therapeutic target ...
Source: Metabolic Brain Disease - Category: Neurology Source Type: research

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Publication date: Available online 31 March 2020Source: Journal of Sound and VibrationAuthor(s): Ge Yan, Hong-Xiang Zou, Sen Wang, Lin-Chuan Zhao, Qiu-Hua Gao, Ting Tan, Wen-Ming Zhang
Source: Journal of Sound and Vibration - Category: Physics Source Type: research
Authors: Matsumura E, Nohara K, Tanaka N, Fujii N, Sakai T Abstract Polypharmacy in elderly persons living at home or in a nursing home is an issue. In the field of dentistry, strategies to reduce polypharmacy must be promoted; however, there is insufficient basic data on medications received by elderly persons with dysphagia living at home or in a nursing home. The subjects were 106 elderly persons with dysphagia living at home or in a nursing home. Based on their medical records, the presence of disease and number/type of drugs being administered were investigated. Stroke, dementia, and hypertension were common. ...
Source: Journal of oral science - Category: Dentistry Tags: J Oral Sci Source Type: research
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Source: Oral health and Preventive dentistry - Category: Dentistry Source Type: research
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Source: Neurophysiologie Clinique - Category: Neuroscience Source Type: research
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Condition:   Stroke Interventions:   Device: Robot-Assisted Hand Training;   Other: Standard Hand Therapy Sponsors:   National University Hospital, Singapore;   National University, Singapore Not yet recruiting
Source: - Category: Research Source Type: clinical trials
Conditions:   Cerebral Small Vessel Diseases;   Cerebral Small Vessel Ischemic Disease;   Lacunar Stroke Interventions:   Diagnostic Test: 7 Tesla Magnetic Resonance Imaging;   Other: Brief Memory and Executive Test Sponsors:   University of Cambridge;   Cambridge University Hospitals NHS Foundation Trust;   Wolfson Brain Imaging Centre Recruiting
Source: - Category: Research Source Type: clinical trials
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Source: Frontiers in Neurology - Category: Neurology Source Type: research
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