Identification of miR390-TAS3-ARF pathway in response to salt stress in Helianthus tuberosus L.

In this study, we isolated and identified MIR390, miR390, TAS3a/b/c, tasiARF-1/2/3 (trans-acting small interfering RNAs influencing Auxin Response Factors) and ARF2/3/4 in Jerusalem artichoke (Helianthus tuberosus L.). Treatment with 100 mM NaCl induced expression of miR390, increased cleavage of TAS3, produced high levels of tasiARFs, and subsequently enhanced cleavage of ARF3/4, which was most likely associated with salt tolerance of the plants. In contrast, treatment with 300 mM NaCl inhibited expression of miR390, attenuated cleavage of TAS3, produced a small amount of tasiARFs, and reduced cleavage of ARF3/4. We proposed that ARF2, one of the targets of tasiARFs, induced under salinity was likely to play an active role in salt tolerance of Jerusalem artichoke. The study of the miR390-TAS3-ARF model in Jerusalem artichoke may broaden our understanding of salt tolerance mechanisms, and provides a theoretical support for further genetic identification and breeding crops with increased tolerance to salt stress. PMID: 32045659 [PubMed - as supplied by publisher]
Source: Gene - Category: Genetics & Stem Cells Authors: Tags: Gene Source Type: research