Dried blood microsamples: suitable as an alternative matrix for the quantification of paracetamol-protein adducts?

Dried blood microsamples: suitable as an alternative matrix for the quantification of paracetamol-protein adducts? Toxicol Lett. 2020 Feb 06;: Authors: Delahaye L, Dhont E, De Cock P, De Paepe P, Stove CP Abstract Paracetamol (acetaminophen, APAP) is the most frequently used analgesic drug worldwide. However, patients in several specific populations can have an increased exposure to toxic APAP metabolites. Therefore, APAP-protein adducts have been proposed as an alternative marker for the assessment of APAP intoxications and as an effective tool to study and steer APAP treatment in patients with an increased risk of APAP-induced liver damage. These adducts have been determined in plasma or serum as a matrix. Blood microsampling allows the determination of a variety of analytes, including protein adducts, in a drop of blood, facilitating convenient follow-up of patients in a home-sampling context, as well as repeated sampling of pediatric patients. We therefore evaluated the use of blood-based volumetric microsamples for the quantification of APAP-protein adducts. Quantitative methods for the determination of APAP-protein adducts in dried blood and dried plasma volumetric absorptive microsamples were developed and validated. Also a preliminary evaluation of pediatric patient dried blood microsamples was conducted. Method validation encompassed the evaluation of selectivity, carry over, calibration model, accuracy and precision, matrix...
Source: Toxicology Letters - Category: Toxicology Authors: Tags: Toxicol Lett Source Type: research