Characterization of endogenous Claudin1 expression, motility and susceptibility to Hepatitis C Virus in CRISPR knock-in cells.

CONCLUSIONS: The Huh7.5.1 TagRFP-CLDN1+/+ edited cell line showed physiological features comparable to that of non-edited cells, but became resistant to HCV infection. Our data also highlights the important impact of the fluorescent protein chosen for endogenous tagging.
SIGNIFICANCE: Although HCV-related studies may not be achieved with these cells, our work provides a novel tool to study the cell biology of tight-junction associated proteins and a potential screening strategy measuring CLDN1 expression levels. This article is protected by copyright. All rights reserved.
PMID: 32034780 [PubMed - as supplied by publisher]

Source: Biology of the Cell - February 6, 2020 Category: Cytology Authors: Clément CMH, Deffieu MS, Dorobantu CM, Baumert TF, Ayala-Nunez NV, Mély Y, Ronde P, Gaudin R Tags: Biol Cell Source Type: research