Metabolic exchange with alkane non-consumer Pseudomonas stutzeri SLG510A3-8 improves the n-alkane biodegradation of the alkane degrader Dietzia sp. DQ12-45-1b.

Metabolic exchange with alkane non-consumer Pseudomonas stutzeri SLG510A3-8 improves the n-alkane biodegradation of the alkane degrader Dietzia sp. DQ12-45-1b. Appl Environ Microbiol. 2020 Feb 07;: Authors: Hu B, Wang M, Geng S, Wen L, Wu M, Nie Y, Tang YQ, Wu XL Abstract Biodegradation of alkanes by microbial communities is ubiquitous in nature. Interestingly, the microbial communities with high hydrocarbon-degrading performances are sometimes composed of not only hydrocarbon degraders but also non-consumers, but the synergistic mechanisms remain unknown. Here, we found that two bacterial strains isolated from Chinese oilfields, Dietzia sp. DQ12-45-1b and Pseudomonas stutzeri SLG510A3-8, had a synergistic effect on hexadecane (C16) biodegradation, even though P. stutzeri could not utilize C16 individually. To gain a better understanding of the roles of the alkane non-consumer P. stutzeri in the C16-degrading consortium, we reconstructed a two-species stoichiometric metabolic model iBH1908, and integrated in silico prediction with the following in vitro validation, a comparative proteomics analysis, and extracellular metabolomic detections. Metabolic interactions between P. stutzeri and Dietzia sp. were successfully revealed for the importance in efficient C16 degradation. In the process, P. stutzeri survived on C16 metabolic intermediates from Dietzia sp., including hexadecanoate, 3-hydroxybutanoate, and α-ketoglutarate. In return,...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research