Evaluation of the biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa on human umbilical cord CD146+ stem cells and stem cell-based decellularized matrix

This study aims to evaluate the CD146+ stem cells obtained from the human umbilical cord and their extracellular matrix proteins on in vitroPseudomonas aeruginosa andStaphylococcus aureus biofilms to understand their possible antimicrobial activity. CD146+ stem cells were determined according to cell surface markers and differentiation capacity. Characterization of the decellularized matrix was done with DAPI, Masson ’s Trichrome staining and proteome analysis. Cell viability/proliferation of cells in co-cultures was evaluated by WST-1 and crystal-violet staining. The effects of cells and decellularized matrix proteins on biofilms were investigated on a drip flow biofilm reactor and their effects on gene expre ssion were determined by RT-qPCR. We observed that CD146/105+ stem cells could differentiate adipogenically and decellularized matrix showed negative DAPI and positive collagen staining with Masson’ s Trichrome. Proteome analysis of the decellularized matrix revealed some matrix components and gro wth factors. Although the decellularized matrix significantly reduced the cell counts ofP. aeruginosa, no significant difference was observed forS. aureus cells in both groups. Supporting data was obtained from the gene expression results ofP. aeruginosa with the significant down-regulation ofrhlR andlasR. ForS. aureus,icaADBC genes were significantly up-regulated when grown on the decellularized matrix.
Source: Cell and Tissue Banking - Category: Stem Cells Source Type: research