Sml1 inhibits DNA repair activity of Rev1 in Saccharomyces cerevisiae during oxidative stress.

Sml1 inhibits DNA repair activity of Rev1 in Saccharomyces cerevisiae during oxidative stress. Appl Environ Microbiol. 2020 Jan 31;: Authors: Yao R, Zhou P, Wu C, Liu L, Wu J Abstract In Saccharomyces cerevisiae, Y family DNA polymerase Rev1 is involved in the repair of DNA damage by translesion DNA synthesis (TLS). In the current study, to elucidate the role of Rev1 in oxidative stress-induced DNA damage in S. cerevisiae, REV1 was deleted and overexpressed; transcriptome analysis of these mutants along with the wild-type strain was performed to screen potential genes that could be associated with REV1 during response to DNA damage. Under 2 mM H2O2 treatment, the deletion of REV1 resulted in a 1.5- and 2.8-fold decrease in the survival rate and mutation frequency, respectively, whereas overexpression of REV1 increased the survival rate and mutation frequency by 1.1- and 2.9-fold, respectively, as compared to the survival rate and mutation frequency of the wild-type strain. Transcriptome and phenotypic analyses identified that Sml1 aggravated oxidative stress in the yeast cells by inhibiting the activity of Rev1. This inhibition was due to the physical interaction between the BRCT domain of Rev1 and the amino acid residues 36-70 of Sml1, the cell survival rate and mutation frequency increased by 1.8 and 3.1-fold, respectively, upon blocking this interaction. We also found that Sml1 inhibited Rev1 phosphorylation under oxidative stress...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research