Cytotoxic Ag(I) and Au(I) NHC-carbenes bind DNA and show TrxR inhibition.
Cytotoxic Ag(I) and Au(I) NHC-carbenes bind DNA and show TrxR inhibition. J Inorg Biochem. 2020 Jan 15;205:110998 Authors: Guarra F, Busto N, Guerri A, Marchetti L, Marzo T, García B, Biver T, Gabbiani C Abstract A silver(I) and a gold(I) complex of the fluorescent N-heterocyclic carbenic (NHC) ligand 1-(9-anthracenylmethyl)-3-(3-trimethylsilyl-2-propynil)-benzimidazol-2-ylidene have been synthesized and characterized. These compounds show cytotoxicity in the micromolar range and higher antiproliferative properties than cisplatin (CDDP) against several tumour cell lines such as SW480 (colon), A549 (lung) and HepG2 (liver). Both metal complexes are successfully internalized by SW480 cells being the silver compound the most accumulated. Subsequently, they were evaluated as inhibitors of the selenoenzyme Thioredoxin reductase (TrxR) and as DNA binders. Fluorescence microscopy confirmed that both protein and DNA binding could be involved in the biological activity of the compounds. The silver carbene was the most effective enzyme inhibitor with an IC50 in the nanomolar range. Also, interaction studies with natural double stranded DNA highlight a strong stabilisation of the double helix after binding to the Ag(I) carbene, indicating its potential suitability as dual-targeting anticancer active molecule. PMID: 31981769 [PubMed - as supplied by publisher]
Publication date: Available online 1 April 2020Source: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsAuthor(s): Florina Zakany, Tamas Kovacs, Gyorgy Panyi, Zoltan Varga
CONCLUSION: Pregnancy outcomes after hysteroscopic placement of a microinsert for hydrosalpinx management before embryo transfer were comparable to those following laparoscopic proximal tubal occlusion or salpingectomy. However, caution is advised regarding microinsert placement for hydrosalpinges before proceeding with assisted reproductive technology. PMID: 32224160 [PubMed - as supplied by publisher]
Authors: Feres M, Retamal-Valdes B, Faveri M, Duarte P, Shibli J, Soares GMS, Miranda T, Teles F, Goodson M, Hasturk H, Van Dyke T, Ehmke B, Eickholz P, Schlagenhauf U, Meyle J, Koch R, Kocher T, Hoffmann T, Kim TS, Kaner D, Figueiredo LC, Doyle H Abstract OBJECTIVE: The selection of proper outcome measures is a critical step in clinical research. Most randomized clinical trials (RCTs) assessing the effects of initial anti-infective periodontal therapies use surrogate outcomes as primary outcome variables, such as mean changes in probing depth (PD) or in clinical attachment. However, these parameters do not reflect...
CONCLUSION: Within the limits of our study, the results of this trial indicate that reconstructive periodontal surgery with PTG offers minimal radiographic defect resolution with no significant improvements in clinical endpoints compared to open flap debridement. PMID: 32224548 [PubMed - in process]
CONCLUSION: P. gingivalis and F. nucleatum inoculation induced significant changes in intestinal inflammation and caecal microbiome. Oral gavage with LGG exerted a protective effect against intestinal inflammation and limited gut microbiome changes associated with P. gingivalis and F. nucleatum. PMID: 32224547 [PubMed - in process]
CONCLUSIONS: Non-surgical periodontal therapy was effective during the first 18 months, but periodontal conditions were worse at 48 months after therapy. The distal sites of abutment teeth with direct retainers presented the worst periodontal conditions. PMID: 32224546 [PubMed - in process]
CONCLUSION: Individuals with DD undergoing PMT presented higher rates of recurrence of periodontitis and tooth loss when compared to individuals without DD. Additionally, the interaction between DD and smoking significantly increased the risk for the recurrence of periodontitis. PMID: 32224545 [PubMed - in process]
Publication date: Available online 31 March 2020Source: Contemporary Educational PsychologyAuthor(s): Eike Wille, Gundula Stoll, Thomas Gfrörer, Jenna Cambria, Benjamin Nagengast, Ulrich Trautwein
Publication date: Available online 31 March 2020Source: Food Quality and PreferenceAuthor(s): Andrijana Mušura Gabor, Bojan Stojnić, David Ban-Ostić
Publication date: Available online 31 March 2020Source: Food Quality and PreferenceAuthor(s): Berta Schnettler, Edgardo Miranda-Zapata, Ligia Orellana, Tino Bech-Larsen, Klaus G. Grunert