Identification of cartilage microbial DNA signatures and associations with knee and hip osteoarthritis

The objective of this study was to characterize the microbial DNA profile of articular cartilage and determine changes associated with osteoarthritis (OA).Methods16s rRNA gene deep sequencing was performed on eroded and intact cartilage samples from knee and hip OA patients and cadaveric controls. Microbial DNA diversity was assessed, groups compared, and metagenomic profiles reconstructed. Confirmation was performed in an independent cohort by clade ‐specific qPCR. Human results were compared to cartilage from OA‐susceptible C57BL6 and OA‐resistant MRL/MpJ mice. Germ‐free C57BL6 mouse cartilage was analyzed as a methodological control.ResultsAlpha diversity was reduced in human OA vs. control (p
Source: Arthritis and Rheumatology - Category: Rheumatology Authors: Tags: FULL LENGTH Source Type: research

Related Links:

As the population ages, falls are becoming one of the leading causes of morbidity and mortality. Joint disease (either osteoarthritis or rheumatoid arthritis) is a well-known predictor of falls, and these medical conditions increase in accordance with the ...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Age: Elder Adults Source Type: news
Conclusion: The findings of this present study may provide new clues for the roles of DEmRNAs and DEmiRNAs in the pathogenesis of OA.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Expression profiling by high throughput sequencing Homo sapiens Source Type: research
AbstractOsteoarthritis (OA) is a progressive and disabling musculoskeletal disease affecting millions of people and resulting in major healthcare costs worldwide. It is the most common form of arthritis, characterised by degradation of the articular cartilage, formation of osteophytes, subchondral sclerosis, synovial inflammation and ultimate loss of joint function. Understanding the pathogenesis of OA and its multifactorial aetiology will lead to the development of effective treatments, which are currently lacking. Two-dimensional (2D) in vitro tissue models of OA allow affordable, high-throughput analysis and stringent c...
Source: Calcified Tissue International - Category: Orthopaedics Source Type: research
AbstractPain is a health issue affecting all populations, regardless of age, gender, economic status, race, or geography. Acute pain is the most common type of pain, with a complex aetiology. Inadequately managed acute pain adversely affects quality of life and imposes significant economic burden. The majority of the available pain-relieving drugs have monomodal mechanisms of analgesia, which necessitates combining drugs with non-redundant mechanisms of action in order to provide adequate pain relief and reduce the side effects from higher doses of individual drugs. In this regard, combining an oral opioid (such as codeine...
Source: Pain and Therapy - Category: Anesthesiology Source Type: research
Synovitis is implicated in the severity and progression of pain and structural pathology of osteoarthritis (OA). Increases in inflammatory or immune cell subpopulations including macrophages and lymphocytes ha...
Source: Arthritis Research and Therapy - Category: Rheumatology Authors: Tags: Research article Source Type: research
Conditions:   Rheumatoid Arthritis - Hand Joint;   Osteoarthritis Hand;   Pain;   Mobility Limitation;   Stiffness of Hand, Not Elsewhere Classified Interventions:   Other: Smartphone Application;   Other: Conventional treatment Sponsors:   University of Seville;   Fundación Pública Andaluza Progreso y Salud Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
ConclusionVisceral fat was associated with an increased risk of musculoskeletal and widespread pain.
Source: Arthritis and Rheumatology - Category: Rheumatology Authors: Tags: FULL LENGTH Source Type: research
Source: Arthritis Research and Therapy - Category: Rheumatology Authors: Tags: Letter Source Type: research
Source: Arthritis Research and Therapy - Category: Rheumatology Authors: Tags: Letter Source Type: research
Source: Arthritis Research and Therapy - Category: Rheumatology Authors: Tags: Letter Source Type: research
More News: Arthritis | Genetics | Microbiology | Osteoarthritis | Rheumatology | Study