Taguchi Design-assisted immobilization of Candida rugosa lipase onto a ternary alginate/nanocellulose/montmorillonite composite: Physicochemical characterization, thermal stability and reusability studies

This study aimed to statistically optimize the covalent immobilization of Candida rugosa lipase (CRL) onto a ternary support comprised of OPFL derived nanocellulose (NC) and montmorillonite (MMT) in alginate (ALG) (CRL-ALG/NC/MMT). The coarser topology and the presence of characteristic spherical globules in the field emission scanning electron micrographs and atomic force micrographs, respectively, supported the existence of CRL on ALG/NC/MMT. In addition, amide peaks at 3478 and 1640 cm-1 in the fourier transform infrared spectra affirmed that CRL was covalently bonded to ALG/NC/MMT. The optimized Taguchi Design-assisted immobilization of CRL onto ALG/NC/MMT (7 h of immobilization, 35℃, pH 5, 7 mg/mL protein loading) gave a production yield of 92.89% of ethyl levulinate (EL), as proven by gas chromatography–mass spectrometric ([M] + m/z 144, C7H12O3), FTIR and nuclear magnetic resonance (CAS-539-88-8) data. A higher optimal reaction temperature (50℃) and the reusability of CRL-ALG/NC/MMT for up to 9 esterification cycles substantiated the appreciable structural rigidification of the biocatalyst by ALG/NC/MMT, which improved the catalytic activity and thermal stability of the lipase.Graphical abstract
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research

Related Links:

In this study, we report the antifungal activity of the 2(5H)-furanone derivative F105, consisting of three pharmacophores, namely chlorinated 2(5H)-furanone, sulfonyl group, and l-menthol moiety. Although exhibiting moderate antifungal activity alone with the minimum inhibitory concentration (MIC) values of 32–256 μg/mL, F105 potentiates the activity of fluconazole and terbinafine with fractional inhibitory concentration index (FICI) values of 0.27–0.50. Thus, 16 μg/mL of F105 reduced the MICs of these antifungals against fluconazole-resistant C. albicans isolates four-fold, achieving sim...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
This study aims to improve the efficacy of a widely used azole, fluconazole by co-delivering it with a Pseudomonas aeruginosa quorum sensing molecule (QSM), N-3-oxo-dodecanoyl-L-homoserine lactone (C12AHL) in a liposomal formulation. C12AHL is known to inhibit C. albicans’ morphological transition and biofilm formation. Four different formulations of liposomes with fluconazole (L-F), with C12AHL (L-H), with fluconazole and C12AHL (L-HF), and a drug-free control (L-C) were prepared using a thin-film hydration followed by extrusion method, and characterised. The effect of liposomes on colonising (90min-24h) and preform...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
oltronieri : The synthesis of metal nanoparticles using plant extracts is a very promising method in green synthesis. The medicinal value of Moringa oleifera leaves and the antimicrobial activity of metallic copper were combined in the present study to synthesize copper nanoparticles having a desirable added-value inorganic material. The use of a hydroalcoholic extract of M. oleifera leaves for the green synthesis of copper nanoparticles is an attractive method as it leads to the production of harmless chemicals and reduces waste. The total phenolic content in the M. oleifera leaves extract was 23.0 ± 0.3 mg...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
A 44-year-old woman presented with lumbar pain (visual analog scale [VAS]  = 9) secondary to adrenocortical carcinoma sclerotic L4-vertebral metastasis. The positron emission tomography-computed tomography hypermetabolic lesion (Fig 1a,d) was unresponsive to radiotherapy or chemotherapy. She refused surgery. Following general anesthesia and aseptic preparation, 2 power d rill-mounted (Aesculap, Center Valley, Pennsylvania) k-wires were inserted in each vertebral pedicle with lateromedial opposite directions, resulting in crossed wires.
Source: Journal of Vascular and Interventional Radiology : JVIR - Category: Radiology Authors: Tags: Extreme IR Source Type: research
Abstract The anti-biofilm activity of an H2O2-generating electrochemical scaffold (e-scaffold) was determined against mono- and tri-species biofilms of methicillin-resistant Staphylococcus aureus, multi-drug resistant Pseudomonas aeruginosa and Candida albicans Significant time-dependent decreases were found in the overall colony-forming units (CFU) of biofilms of all three mono- as well as the tri-species forms. Confocal laser scanning microscopy (CLSM) showed dramatic reductions in fluorescence intensities of biofilm matrix protein and polysaccharide components of e-scaffold-treated biofilms. The described e-sca...
Source: Antimicrobial Agents and Chemotherapy - Category: Microbiology Authors: Tags: Antimicrob Agents Chemother Source Type: research
Publication date: Available online 15 January 2020Source: Biochimica et Biophysica Acta (BBA) - BiomembranesAuthor(s): Gopal Pandit, Karishma Biswas, Suvankar Ghosh, Swapna Debnath, Anil P. Bidkar, Priyadarshi Satpati, Anirban Bhunia, Sunanda ChatterjeeAbstractThe widespread abuse of antibiotics has led to the use of antimicrobial peptides (AMPs) as a replacement for the existing conventional therapeutic agents for combating microbial infections. The broad-spectrum activity and the resilient nature of AMPs has mainly aggrandized their utilization. Here, we report the design of non-toxic, non-hemolytic and salt tolerant und...
Source: Biochimica et Biophysica Acta (BBA) Biomembranes - Category: Biochemistry Source Type: research
CONCLUSIONS: The biofilm formed in the three materials used in the manufacture of abutments and prostheses showed no major differences, being far less dense than in the resins. Two clinical recommendations can be made: to avoid the presence of resins in the subgingival area of implant prostheses and to design prostheses placing cobalt-chromium alloy/ceramic or titanium/ceramic junctions as far as possible from implants. PMID: 31880295 [PubMed - as supplied by publisher]
Source: Medicina Oral, Patologia Oral y Cirugia Bucal - Category: ENT & OMF Tags: Med Oral Patol Oral Cir Bucal Source Type: research
You know probiotics can be good for your gut, but does your vagina need one too? You might think so, based on probiotic marketing these days. Probiotics are in everything from drinks to pills and powders, and in many cases, are being promoted as a means of improving your vaginal health. Women seem to be listening, says Dr. Caroline Mitchell, assistant professor of obstetrics, gynecology, and reproductive biology at Harvard Medical School. Vaginal probiotic supplements are hugely popular. This includes both probiotic pills and suppository capsules that are inserted into the vagina using an applicator. But evidence of effect...
Source: Harvard Health Blog - Category: Consumer Health News Authors: Tags: Health Sexual Conditions Vitamins and supplements Women's Health Source Type: blogs
This study assessed the antimicrobial and antibiofilm activity of these peptides. The antifungal activity of kyotorphins was determined in representative strains of Candida species, including C. albicans ATCC 10231, C. krusei ATCC 6258, and six clinical isolates—C. dubliniensis 19-S, C. glabrata 217-S, C. lusitaniae 14-S, C. novergensis 51-S, C. parapsilosis 63, and C. tropicalis 140-S—obtained from the oral cavity of HIV-positive patients. The peptides were synthesized by standard solution or solid-phase synthesis, purified by RP-HPLC (purity>95%), and characterized by nuclear magnetic resonance. The result...
Source: Fungal Biology - Category: Biology Source Type: research
Abstract The present study deals with novel synthesizing method of TEMPO oxidized cellulose (extracted from bagasse) (TOC) amino acids (l-phenyl alanine (Phe) and l-tryptophan (Trp)) nano-composites as potential antimicrobial biocompatible agents. The produced nanocomposites were characterized via Fourier transform Infrared (FT-IR) spectroscopy, thermal analysis (TGA and DTGA), scanning electron microscope(SEM), and transmission electron microscope (TEM) which approved that the synthesis of composites in nano-scale in spherical shape with average particle size 72 and 44.37 nm for l-phenylalanine composite (Phe-T...
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
More News: Biotechnology | Candida | Nanotechnology | Nursing | PET Scan | Statistics | Study