Recombinant adenovirus carrying a core neutralizing epitope of porcine epidemic diarrhea virus and heat-labile enterotoxin B of Escherichia coli as a mucosal vaccine

In this study, we generated a recombinant adenovirus vaccine encoding the heat-labile enterotoxin B (LTB) and the core neutralizing epitope (COE) of PEDV (rAd-LTB-COE). The fusion protein LTB-COE was successfully expressed by the recombinant adenovirus in HEK293 cells, and the immunogenicity of the vaccine candidate was assessed in BALB/c mice and piglets. Three intramuscular or oral vaccinations with rAd-LTB-COE at two-week intervals induced robust humoral and mucosal immune responses. Moreover, a cell-mediated immune response was promoted in immunized mice, and the neutralizing antibody inhibited both the vaccine strain and the emerging PEDV isolate. Immunization experiments in piglets revealed that rAd-LTB-COE was immunogenic and induced good immune responses in piglets. Further studies are required to evaluate the efficacy of rAd-LTB-COE against a highly virulent PEDV challenge.
Source: Archives of Virology - Category: Virology Source Type: research

Related Links:

Authors: Wieland U, Oellig F, Kreuter A Abstract Anal intraepithelial neoplasia (AIN) and 89-100% of anal cancers are caused by persistent infections with high-risk (HR) human papillomaviruses (HPV). In HIV-positive patients, anal HPV infection and AIN are very common and these patients have a greatly increased risk of developing anal cancer. However, a continuous increase in the incidence of anal cancer has also been observed in the general population in recent decades. AIN can clinically present in diverse manners. In HIV-positive patients AIN can be hidden in condylomas. Furthermore, 3-14% of high-grad...
Source: Der Hautarzt: Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete - Category: Dermatology Tags: Hautarzt Source Type: research
This study highlights the need for a better understanding of the complex ecology and dynamics of fluoroquinolone resistance in C. jejuni in poultry environment and warrants an examination of fluoroquinolone-resistant C. jejuni strains recovered from the natural broiler chicken environment.
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Publication date: Available online 19 February 2020Source: Journal of Molecular BiologyAuthor(s): Madhu Nagaraj, Mumdooh Ahmed, Jeppe Lyngsø, Brian Stougaard Vad, Andreas Bøggild, Anne Fillipsen, Jan Skov Pedersen, Daniel Erik Otzen, Ümit AkbeyAbstractProtein fibrillation is traditionally associated with misfolding, loss of functional phenotype and gain of toxicity in neurodegenerative diseases. However, many organisms exploit fibrils in the form of functional amyloids (FA), as seen in bacteria such as E. coli, Salmonella, Bacillus and Pseudomonas. Here, we provide structural information and mechanistic ...
Source: Journal of Molecular Biology - Category: Molecular Biology Source Type: research
In this study, we sought to investigate whether SH0165 (serovar 5, high-virulent strain) and HN0001 (serovar 6, non-virulent strain) infection induces autophagy and the specific role of autophagy in bacterial invasion and inflammation during H. parasuis infection. Moreover, we explored the mechanism underlying autophagy regulated inflammation through inflammatory signaling cascades during H. parasuis infection. This observation could provide useful information for further understanding the role of autophagy in H. parasuis infection and improve our knowledge of new strategies against this pathogen. Materials and Methods B...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
In this study, the diagnostic accuracy of InGenius Gastrointestinal Viral (GV) Elite Panel, a newly developed one-step multiplex real-time RT-PCR assay simultaneously detecting rotavirus, adenovirus and astrovirus, was evaluated retrospectively analyzing an archival collection of 128 stool samples of children hospitalized with acute gastroenteritis. The overall sensitivity and specificity for the GV assay was 100% and 96.2% for rotavirus, 96.9% and 100% for astrovirus, 100% and 100% for adenovirus, respectively. The InGenius GV assay showed a high concordance with the reference methods and was able to detect all tested gen...
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research
In this study, the presence of PAdV was investigated in fecal samples collected from piglets with and without diarrhea from 31 commercial pig farms in northern Thailand. A total of 781 fecal specimens (516 from diarrheic piglets and 265 from non-diarrheic piglets) were screened for adenovirus using nested-PCR. Initial screening both in diarrheic and non-diarrheic piglets showed the overall prevalence of PAdV infection in piglets at 16.9% (132/781). Co-infection with PRVA was found in 24 out of 132 (18.2%) PAdV positive cases whereas PAdV mono-infection was observed at 81.8% (108/132). The prevalence of PAdV infection in di...
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research
Authors: Chen S, Tian X Abstract Human mastadenovirus (HAdVs) can cause a broad spectrum of diseases in both children and adults, including acute respiratory infection, gastroenteritis, epidemic keratoconjunctivitis. Populations susceptible to adenovirus infection include children, immunocompromised patients and military recruits. To date, seven species (A-G) including more than 79 genotypes have been characterized, of which HAdV-B3, B4, B7 and the recently reemerged types 14 and 55 often lead to severe pneumonia. The live oral enteric-coated adenovirus type 4 and 7 vaccine, which was approved for use in US militar...
Source: Journal of Thoracic Disease - Category: Respiratory Medicine Tags: J Thorac Dis Source Type: research
Adenoviruses are endemic, primarily causing respiratory and ocular (pharyngoconjunctival fever) symptoms and gastroenteritis infections in infants and children. They also cause epidemic keratoconjunctivitis, pneumonia in military recruits and severe, life-threatening infections in transplant recipients. The hardy non-enveloped virus resists environmental degradation and is transmitted by aerosolized droplets, fomites and faecal-oral spread. Outbreaks occur in medical, day care, military and other facilities where people live in close proximity.
Source: Medicine - Category: Internal Medicine Authors: Tags: Viral infections Source Type: research
Authors: Jang J, Lee YJ, Kim JS, Chung JY, Chang S, Lee K, Choe BH, Hong SJ, Song JS, Park KY Abstract We investigated the adenoviral etiology and seasonal epidemic trends in intussusception and each adenoviral subgroup. Also we confirmed whether we can use the adenovirus data of Acute Infectious Agents Laboratory Surveillance Report (AIALSR) as an epidemic predictor of intussusception. Patients with intussusception (n = 126),
Source: Journal of Korean Medical Science - Category: Biomedical Science Tags: J Korean Med Sci Source Type: research
Authors: Bayrakdar F, Coşgun Y, Salman Atak T, Karademir H, Korukluoğlu G Abstract Although adenoviruses (AdVs) generally cause upper respiratory tract infections, conjunctivitis/epidemic keratoconjunctivitis, gastroenteritis and pneumonia, they can lead to the involvement of central nervous system. Acute flaccid paralysis (AFP) is a type of seizure, characterized by rapid and sudden onset of extreme weakness in hands and feet, including (less frequently) weakness of respiratory and swallowing, representing with decreased muscle tone, especially in children below 15-year-old. The major viral cause of AFP is polio...
Source: Mikrobiyoloji Bulteni - Category: Microbiology Tags: Mikrobiyol Bul Source Type: research
More News: Adenoviruses | Epidemics | Epidemiology | Gastroenteritis | Study | Vaccines | Virology