A Neutralization scFv Antibody against IL-1β Isolated from a NIPA-based Bacterial Display Library.

Conclusions: In this study, it is the first time to use the NIPA-based bacteria display system to construct and screen the combinatorial scFv library. Three scFvs against hIL-1β were obtained from the scFv library of the immunized mice. </p><p> Prokaryotically expressed and purified scFvs demonstrate binding ability with hIL-1β. Among the three clones, The MTT test suggests that scFv-20 is a neutralization antibody against hIL-1β. The study provides a lead candidate for further development of small molecule therapeutic antibodies for treatment of RA. </p><p> PMID: 24016270 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Biotechnology - Category: Biotechnology Authors: Tags: Curr Pharm Biotechnol Source Type: research