A Clinic-Based Direct Real-time Fluorescent Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Influenza Virus.

A Clinic-Based Direct Real-time Fluorescent Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Influenza Virus. J Virol Methods. 2019 Dec 12;:113801 Authors: Ngan LT, Takayama I, Binh NG, Phuong TT, Tuong Van VT, Vuong BM, Co DX, Dung LT, Phuong PT, Cuong DD, Thach PT, Van Thanh D, Phuong Thuy PT, Chau NQ, Tuan DQ, Takasaki J, Semba S, Odagiri T, Nakajima N, Kageyama T Abstract Seasonal influenza virus causes acute respiratory tract infections, which can be severe in children and the elderly. At present, rapid influenza diagnostic tests (RIDTs) are popular at clinical sites because they enable early diagnosis and avoid unnecessary use of antibiotics; in addition, high risk patients with underlying disease can be given antiviral drugs. However, the sensitivity and specificity of some of those tests are relatively poor. To overcome these problems, nucleic acid-based molecular point-of-care tests have been developed; however, they are significantly more expensive than RIDTs. Previously, the authors developed real-time reverse transcription loop-mediated isothermal amplification (rRT-LAMP) assays using a quenching primer to detect influenza viruses. However, the assay is limited to laboratory use because it requires a nucleic acid purification step and preparation of reaction mixtures on ice. Therefore, the authors developed and validated direct rRT-LAMP assays that require no nucleic acid purification steps using com...
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research