Comparative genome and transcriptome analysis of the nematode-trapping fungus Duddingtonia flagrans reveals high pathogenicity during nematode infection

Publication date: Available online 14 December 2019Source: Biological ControlAuthor(s): Wei Zhang, Dandan Liu, Zhichao Yu, Bin Hou, Yaru Fan, Zehao Li, Shijie Shang, Yidan Qiao, Jiangtao Fu, Jiekang Niu, Bin Li, Kexin Duan, Xiaoye Yang, Rui WangAbstractDuddingtonia flagrans is a nematode-trapping fungus that is widely used to control parasitic nematodes in livestock. To investigate the features and advantages of D. flagrans for nematode control at the molecular level, the genome and transcriptome of D. flagrans were sequenced and studied through a comparative analysis. The genome of D. flagrans had a size of 36.3 Mb and contained a higher ratio of repetitive sequences, which revealed its slow evolution. Comparative genomics analysis revealed the genome of D. flagrans to be relatively conserved compared to eight sequenced fungal genomes. Genes related to nematode trapping, virulence and pathogenicity were expanded in the D. flagrans genome, such as PHI genes, cytochrome P450 genes and various secretory protein genes. Remarkably, D. flagrans possessed a significant variety and abundance of putative proteases, while carbohydrate degradation-related genes were rare. Based on transcriptional profiling of different nematode trapping stages (0 h, 12 h and 48 h), the genes for some crucial secreted enzymes were up-regulated during nematode trapping process, including Zn(2)-C6-type transcription factor, chitinases, serine proteases and lectin-coding genes. The draft gen...
Source: Biological Control - Category: Biology Source Type: research

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Publication date: Available online 20 January 2020Source: Veterinary ParasitologyAuthor(s): Djordje S. Marjanović, Nemanja Zdravković, Mirjana Milovanović, Jelena Nedeljković Trailović, Alan P. Robertson, Zoran Todorović, Saša M. TrailovićAbstractThe neuromuscular system of parasitic nematodes has proven to be an efficient pharmacological target for antihelmintics. Some of the most frequently used antiparasitic drugs are agonists or antagonists of nicotinic acetylcholine receptors (nAChRs). The antinematodal mechanism of action of carvacrol involves the inhibition of parasite muscle contraction. We have exami...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
J. Mater. Chem. A, 2020, Advance Article DOI: 10.1039/C9TA07410D, PaperMarco Valenti, Yorick Bleiji, Javier Blanco Portals, Loreta A. Muscarella, Mark Aarts, Francesca Peiro, Sonia Estrade, Esther Alarc ón-Lladó InAs crystal grain size reduction by potential-enabled parasitic gas-evolution reactions in the electrochemically-mediated growth of InAs. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - J. Mater. Chem. latest articles - Category: Chemistry Authors: Source Type: research
Not in person, as both are rather historical, but when in Paris, I encountered:Dr Alphonse LaveranandDr Paul GachetDr Laveran has a square named after him, adjacent to the Ecole de Val de Grace, a school of military medicine, where he worked after serving in the army as a physician, and where his father had held the same post.   Paris is good at telling you who a street is named after, so the street sign tells you he was the first French winner of theNobel Prize for Medicine or Physiology.  He won it for identifying both malaria and sleeping sickness as caused by parasites.Dr Gachet has aportrait in the...
Source: Browsing - Category: Databases & Libraries Source Type: blogs
Publication date: Available online 7 January 2020Source: Nano EnergyAuthor(s): Xinming Fan, Guorong Hu, Bao Zhang, Xing Ou, Jiafeng Zhang, Wengao Zhao, Haiping Jia, Lianfeng Zou, Peng Li, Yong YangAbstractNi-rich layered oxides are extensively employed as a promising cathode material in lithium ion batteries (LIBs) due to their high energy density and reasonable cost. However, the hiearchical strcuture of secondary particles with grain boundaries inevitably induces the structural collapse and severe electrode/electrolyte interface parasitic reactions as the intergranular crack arises from the anisotropic shrink and expansi...
Source: Nano Energy - Category: Nanotechnology Source Type: research
Contributors : Paul Herrera ; Lisa Schuster ; Cecilia Wentrup ; Lena K önig ; Thomas Kempinger ; Hyunsoo Na ; Jasmin Schwarz ; Stephan Köstlbacher ; Florian Wascher ; Markus Zojer ; Thomas Rattei ; Matthias HornSeries Type : Expression profiling by high throughput sequencingOrganism : Parachlamydia acanthamoebae UV-7Symbiosis is a ubiquitous phenomenon in nature, and these inter-species interactions have a massive impact on organisms, shaping the world around us today. The relationship between the partners in microbial symbioses have been described as existing along a parasitism-mutualism continuum, and the dynam...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Parachlamydia acanthamoebae UV-7 Source Type: research
Publication date: Available online 18 January 2020Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Serene Yu, Joseph Modarelli, John M. Tomeček, Justin T. French, Clayton Hilton, Maria D. Esteve-GasentAbstractDetermining which wildlife hosts are involved in the enzootic cycles of tick-borne diseases (TBD) enables enhanced surveillance and risk assessment of potential transmission to humans and domestic species. Currently, there is limited data to indicate which tick-borne pathogens (TBP) can infect coyotes. Additionally, limited surveillance data for white-tailed deer (WTD) in south Texas i...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
Publication date: Available online 10 January 2020Source: Journal of Molecular BiologyAuthor(s): Sarah E. Allen, Mariusz NowackiAbstractCiliates are an interesting model system for investigating diverse functions of noncoding RNAs, especially in genome defence pathways. During sexual development, the ciliate somatic genome undergoes massive rearrangement and reduction through removal of transposable elements and other repetitive DNA. This is guided by a multitude of noncoding RNAs of different sizes and functions, the extent of which is only recently becoming clear. The genome rearrangement pathways evolved as a defence ag...
Source: Journal of Molecular Biology - Category: Molecular Biology Source Type: research
Publication date: Available online 18 January 2020Source: Journal of Electroanalytical ChemistryAuthor(s): Tim Tichter, Jonathan Schneider, Duc Nguyen Viet, Alvaro Diaz Duque, Christina RothAbstractThin film rotating-ring disc electrode (RRDE) technique is exploited to quantify the parasitic hydrogen evolution reaction (HER) competing with the desired V3+-reduction at surface modified carbon nanoparticles for application as electrocatalysts in the negative half-cell of vanadium redox-flow batteries (VRFB). Carbon based electrode materials are derived from standard Vulcan XC-72 carbon, treated by chemical surface etching te...
Source: Journal of Electroanalytical Chemistry - Category: Chemistry Source Type: research
Authors: Lizardo K, Ayyappan JP, Ganapathi U, Dutra WO, Qiu Y, Weiss LM, Nagajyothi JF Abstract Chagas disease is caused by Trypanosoma cruzi which is endemic in Latin America. T. cruzi infection results in a latent infection with approximately a third of latently infected patients developing chronic Chagas cardiomyopathy (CCM). CCM is a common cause of cardiomyopathy in endemic regions and has a poor prognosis compared to other cardiomyopathies. The factors responsible for the transition from the asymptomatic indeterminate latent stage of infection to CCM are poorly understood. Our previous studies demonstrated th...
Source: Disease Markers - Category: Laboratory Medicine Tags: Dis Markers Source Type: research
Publication date: Available online 18 January 2020Source: Veterinary ParasitologyAuthor(s): Emily Kate Francis, Janina McKay-Demeler, Nichola Eliza Davies Calvani, Denise McDonell, Jan ŠlapetaAbstractIn Australia, Cooperia spp. are often overshadowed by parasites believed to be more pathogenic production-limiting nematodes. A rise in anthelmintic resistance and reports of reduced growth rates attributed to infection with Cooperia spp. in Europe increases the need to be able to monitor the presence of C. pectinata, C. punctata and C. oncophora in Australian cattle. Here, we present the first molecular confirmation of...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
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