Single-Tube Multiplex Polymerase Chain Reaction for the Detection of Genes Encoding Enterobacteriaceae Carbapenemase.

In conclusion, the combination of multiplex PCR primers and QIAGEN Multiplex PCR Plus Kit was used to determine the best performance for the rapid and efficient screening of carbapenemase genes in Enterobacteriaceae. The assay had an overall sensitivity of 100% and a specificity of 100%. This PCR assay will compensate for the limitations of phenotypic testing, such as antimicrobial susceptibility testing and the modified carbapenem inactivation method, among others, in clinical and public health settings.
PMID: 31787735 [PubMed - as supplied by publisher]

Source: Japanese Journal of Infectious Diseases - November 28, 2019 Category: Infectious Diseases Authors: Watahiki M, Kawahara R, Suzuki M, Aoki M, Uchida K, Matsumoto Y, Kumagai Y, Noda M, Masuda K, Fukuda C, Harada S, Senba K, Suzuki M, Matsui M, Suzuki S, Shibayama K, Shinomiya H Tags: Jpn J Infect Dis Source Type: research