The effect of Padma-hepaten herbal combination on the caries-inducing properties of Streptococcus mutans on orthodontic surfaces

Publication date: Available online 4 December 2019Source: Journal of Herbal MedicineAuthor(s): Farkash Yosi, Feldman Mark, Ginsburg Isaac, Shalish Miriam, Steinberg DoronAbstractDental biofilm is recognized as the primary cause of oral infections, caries being the most common. Orthodontic appliances might increase the risk for dental caries and white spot lesions. Phytomedicinal methods were shown to inhibit dental biofilm formation. The goal of this study was to examine whether Padma hepaten (PH), a polyphenolic formula derived from traditional Tibetan medicine, could inhibit the caries-inducing properties of S. mutans. Biofilms of S. mutans were grown in the presence of PH. Formation of biofilms was tested spectrophotometrically. Exopolysaccharides (EPS) secretion was quantified using confocal scanning laser microscopy. Gene expression of caries-related genes was tested using qRT-PCR. Development of biofilm was also tested on an orthodontic surface (Essix) to assess biofilm inhibition ability on such orthodontic appliances. PH had a dose-dependent inhibition on biofilm formation, with no inhibition on planktonic bacterial growth. The minimal biofilm concentration needed to inhibit the formation by 50 % was 0.16 mg/ml PH. This concentration inhibited EPS secretion by 88 %. A strong reduction was found in the expression of gtfB, gtfC, ftf and brpA caries associated genes. These results demonstrate the inhibitory effect of natural PH polyphenolic extraction on S. mutans...
Source: Journal of Herbal Medicine - Category: Complementary Medicine Source Type: research

Related Links:

Abstract Objective: To evaluate the association of body mass index (BMI) and gross national income (GNI) per capita with dental caries experience in children at population levels.Methods: This ecological study used global data of decay, missing, and filled teeth (DMFT), BMI, and GNI. DMFT data of 12 years old children from 117 countries were obtained from the World Health Organization. BMI data of children and adolescents from the same 117 countries were retrieved from the NCD Risk Factor Collaboration and GNI per capita from the World Bank. ANOVA test, Pearson's correlation coefficient (r), and multivariab...
Source: Acta Odontologica Scandinavica - Category: Dentistry Authors: Tags: Acta Odontol Scand Source Type: research
Abstract The present study aimed at investigating the influence of norspermidine on the formation of dual-species biofilms composed of Streptococcus mutans (S. mutans) and Streptococcus sanguinis (S. sanguinis). Crystal violet assay was conducted to assess the formation of single-species biofilms of S. mutans and S. sanguinis, and the growth curve was carefully observed to monitor the growth of these two species of bacteria. Fluorescence in situ hybridization (FISH) and MTT array were used to analyze the composition and metabolic activity of the dual-species biofilms, respectively. Extracellular polysaccharides (E...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
Conclusions: There are many behavioural and socioeconomic factors that associate with the occurrence of both periodontal disease and dental caries. These factors also increase the risk of individuals having these diseases simultaneously. PMID: 31686553 [PubMed - as supplied by publisher]
Source: Acta Odontologica Scandinavica - Category: Dentistry Authors: Tags: Acta Odontol Scand Source Type: research
Conclusions: The findings suggest a U-shaped relationship between severe dental caries and both ends of anthropometric measures among pre-school Chinese children. The findings highlight the importance of integrating oral and general health promotion policies. Primary health care providers are encouraged to incorporate dental screening, counselling and referral for treatment for severe caries to promote appropriate growth and overall health of children. PMID: 31648582 [PubMed - as supplied by publisher]
Source: Acta Odontologica Scandinavica - Category: Dentistry Authors: Tags: Acta Odontol Scand Source Type: research
This study found that The Canary System was more accurate than Bitewing Radiographs and Cone Beam CT in detecting caries on the gingival floor beneath composite restorations.  The sensitivity and specificity data (table on the right) indicates that The Canary System could find 89% of the caries where the other devices found 40% of the lesions.One of the major reasons for replacing restorations is tooth decay that develops around the edges of the filling.  This study found that “Radiographs though valuable in the detection of advanced caries lesions, are less sensitive for early or recurrent lesions under re...
Source: Dental Technology Blog - Category: Dentistry Source Type: news
Dental caries and periodontitis are the most common oral disease of all age groups, affecting billions of people worldwide. These oral diseases are mostly associated with microbial biofilms in the oral cavity. Streptococcus gordonii, an early tooth colonizing bacterium and Candida albicans, an opportunistic pathogenic fungus, are the two abundant oral microbes that form mixed biofilms with augmented virulence, affecting oral health negatively. Understanding the molecular mechanisms of the pathogen interactions and identifying non-toxic compounds that block the growth of biofilms are important steps in the development of ef...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusions: This investigation suggests a negative association between bone mineral status and oral health condition regarding DMFT and number of missing teeth. It could be concluded that inadequate bone mass could coexist with higher number of missing teeth or a worse oral health condition; nevertheless, further studies are required to assess these relationships precisely.Keywords: Absorptiometry, bone density: Oral health, dental caries, dual ‑energy X‑ray absorptiometry scan, osteoporosis, periodontal diseases
Source: International Journal of Preventive Medicine - Category: International Medicine & Public Health Source Type: research
Streptococcus mutans is considered the most relevant bacteria in the transition of non-pathogenic commensal oral microbiota to biofilms which contribute to the dental caries process. The present study aimed to evaluate the antimicrobial activity of a natural plant product, cinnamaldehyde against S. mutans biofilms. Minimum inhibitory concentrations (MIC), minimal bactericidal concentration (MBC), and growth curves were determined to assess its antimicrobial effect against planktonic S. mutans. The biofilm biomass and metabolism with different concentrations of cinnamaldehyde and different incubation time points were assess...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusions: These findings call attention to the need to implement effective public policies directed at avoiding tooth decay in pregnancy and in the postpartum period. PMID: 31519125 [PubMed - as supplied by publisher]
Source: Acta Odontologica Scandinavica - Category: Dentistry Authors: Tags: Acta Odontol Scand Source Type: research
ConclusionOur study showed that hypoplastic AI patients have (i) a low DMFT index, (ii) an alkaline pH of saliva enriched with Bacillus spp, Enterococcus faecalis and Enterococcus faecium and (iii) dental tissues more easily invaded by Lactobacilli than Streptococci. The combination of these bacteria seems to give AI patients protection against dental caries.
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
More News: Child Development | Children | Complementary Medicine | Genetics | Herbs | PET Scan | Study | Tooth Decay