Inhibition of Autophagy of Cajal Mesenchymal Cells by Gavage of Tong Bian Decoction based on the Rat Model of Chronic Transit Constipation

In this study, the transport function of colon of rats in each group was detected by activated carbon method, and the number of fecal residues in the colon was observed. The mRNA expression of c-kit gene in intestinal tissue of rat was detected by real-time quantitative polymerase chain reaction (RT-qPCR). In addition, the changes of ICC in rats treated with different drugs were detected by immunohistochemical method. The results revealed that in the tong bian decoction gavage group, the water content in the feces of rats was remarkably increased (P<0.05), the amount of residual feces in the colon was remarkably reduced (P<0.01), the percentage of carbon powder propulsion in small intestine was remarkably increased (P<0.01), the staining area of ICC positive cells in colon tissue was remarkably increased (P<0.05), and the expression of c-kit mRNA was remarkably increased (P<0.01). It can be concluded that the tong bian decoction could effectively enhance the colon transport function in the rat model of CTC. This laxative mechanism promotes the regeneration and repair ability of ICC by inhibiting the autophagy of ICC, and provides power for the large intestine, so as to achieve the free movement of the bowels. Therefore, the results of this study have certain guiding meaning for the treatment of CTC with traditional Chinese medicine.
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research