Systems toxicology assessment of a representative e-liquid formulation using human primary bronchial epithelial cells

Publication date: Available online 25 November 2019Source: Toxicology ReportsAuthor(s): Diego Marescotti, Carole Mathis, Vincenzo Belcastro, Patrice Leroy, Stefano Acali, Florian Martin, Rémi Dulize, David Bornand, Dariusz Peric, Emmanuel Guedj, Laura Ortega Torres, Matteo Biasioli, Matthieu Fuhrimann, Estela Fernandes, Felix Frauendorfer, Ignacio Gonzalez Suarez, Davide Sciuscio, Nikolai V. Ivanov, Manuel C. Peitsch, Julia HoengAbstractThe development of reduced-risk products aims to provide alternatives to cigarettes that present less risk of harm for adult smokers. Responsible use of flavoring substances in these products may fulfill an important role in product acceptance. While most flavoring substances used in such products are also used by the food industry and are considered safe when ingested, their impact when inhaled may require further assessment. To aid in such an assessment, a three-step approach combining real-time cellular analysis, phenotypic high-content screening assays, and gene expression analysis was developed and tested in normal human bronchial epithelial cells with 28 flavoring substances commonly used in e-liquid formulations, dissolved individually or as a mixture in a base solution composed of propylene glycol, vegetable glycerin, and 0.6% nicotine. By employing this approach, we identified individual flavoring substances that potentially contribute greatly to the overall mixture effect (citronellol and alpha-pinene). By assessing modified mixture...
Source: Toxicology Reports - Category: Toxicology Source Type: research