Cancers, Vol. 11, Pages 1846: Mechanistic Signatures of Human Papillomavirus Insertions in Anal Squamous Cell Carcinomas
Cancers, Vol. 11, Pages 1846: Mechanistic Signatures of Human Papillomavirus Insertions in Anal Squamous Cell Carcinomas
Cancers doi: 10.3390/cancers11121846
Authors:
Adeline Morel
Cindy Neuzillet
Maxime Wack
Sonia Lameiras
Sophie Vacher
Marc Deloger
Nicolas Servant
David Veyer
Hélène Péré
Odette Mariani
Sylvain Baulande
Roman Rouzier
Maud Kamal
Elsy El Alam
Emmanuelle Jeannot
Alain Nicolas
Ivan Bièche
Wulfran Cacheux
The role of human papillomavirus (HPV) in anal squamous cell carcinoma (ASCC) carcinogenesis has been clearly established, involving the expression of viral oncoproteins and optional viral DNA integration into the host genome. In this article, we describe the various mechanisms and sites of HPV DNA insertion and assess their prognostic and predictive value in a large series of patients with HPV-positive ASCC with long-term follow-up. We retrospectively analyzed 96 tumor samples from 93 HPV-positive ASCC patients using the Capture-HPV method followed by Next-Generation Sequencing, allowing determination of HPV genotype and identification of the mechanisms and sites of viral genome integration. We identified five different mechanistic signatures of HPV insertions. The distribution of HPV signatures differed from that previously described in HPV-positive cervical carcinoma (p < 0.001). In ASCC samples, the HPV genome more frequently remained in episomal form (45.2%). The most common signature of HPV insertio...
Source: Cancers - Category: Cancer & Oncology Authors: Adeline Morel Cindy Neuzillet Maxime Wack Sonia Lameiras Sophie Vacher Marc Deloger Nicolas Servant David Veyer H élène Péré Odette Mariani Sylvain Baulande Roman Rouzier Maud Kamal Elsy El Alam Emmanuelle Jeannot Alain Nicolas Ivan Bi èche Wulfran C Tags: Article Source Type: research
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