Characterisation of the developing heart in a pressure overloaded model utilising RNA sequencing to direct functional analysis.

Characterisation of the developing heart in a pressure overloaded model utilising RNA sequencing to direct functional analysis. J Anat. 2019 Nov 14;: Authors: Parnall M, Perdios C, Pang KL, Rochette S, Loughna S Abstract Cardiogenesis is influenced by both environmental and genetic factors, with blood flow playing a critical role in cardiac remodelling. Perturbation of any of these factors could lead to abnormal heart development and hence the formation of congenital heart defects. Although abnormal blood flow has been associated with a number of heart defects, the effects of abnormal pressure load on the developing heart gene expression profile have to date not clearly been defined. To determine the heart transcriptional response to haemodynamic alteration during development, outflow tract (OFT) banding was employed in the chick embryo at Hamburger and Hamilton stage (HH) 21. Stereological and expression studies, including the use of global expression analysis by RNA sequencing with an optimised procedure for effective globin depletion, were subsequently performed on HH29 OFT-banded hearts and compared with sham control hearts, with further targeted expression investigations at HH35. The OFT-banded hearts were found to have an abnormal morphology with a rounded appearance and left-sided dilation in comparison with controls. Internal analysis showed they typically had a ventricular septal defect and reductions in the myocardial wall ...
Source: Journal of Anatomy - Category: Anatomy Authors: Tags: J Anat Source Type: research