Effect of Iron Deficiency on a Murine Model of Smoke-Induced Emphysema.

Effect of Iron Deficiency on a Murine Model of Smoke-Induced Emphysema. Am J Respir Cell Mol Biol. 2019 Nov 14;: Authors: Sato K, Inoue S, Igarashi A, Tokairin Y, Yamauchi K, Kimura T, Nishiwaki M, Nemoto T, Nakano H, Sato M, Machida H, Yang S, Minegishi Y, Furuyama K, Watanabe M, Shibata Y Abstract Smoking is a major risk factor for chronic obstructive pulmonary disease (COPD). Smoking susceptibility is important for the onset and development of COPD. We previously reported an association between serum iron levels and pulmonary function in male smokers. However, the mechanism governing smoking susceptibility in relation to iron deficiency is unclear; this study aimed to elucidate this mechanism. C57BL/6 male mice were fed an iron-deficient or normal diet, and then exposed to cigarette smoke. Bronchoalveolar lavage (BAL), histological analysis, and pulmonary function tests were performed after cigarette smoke exposure. Human alveolar type-2 epithelial A549 cells were treated with an iron chelator. Subsequently, A549 cells were exposed to cigarette smoke extract (CSE). In mice exposed to cigarette smoke for 2 weeks, concentration of alveolar macrophages in the BAL fluid recovered from iron-deficient mice was significantly higher than in normal diet mice. Interleukin-6 and monocyte chemotactic protein-1 levels in the BAL fluid increased significantly from baseline in iron-deficient mice, but not in normal diet mice. In mice exposed to ...
Source: Am J Respir Cell Mol... - Category: Respiratory Medicine Authors: Tags: Am J Respir Cell Mol Biol Source Type: research