Hypericum erectum alcoholic extract inhibits Toxoplasma growth and Entamoeba encystation: an exploratory study on the anti-protozoan potential

AbstractHypericum erectum is an important ethnobotanical medicine in East Asian tradition. To explore the anti-parasitic potential ofH. erectum, inhibitory effects on the growth of intracellular parasiteToxoplasma and on the encystation of intestinal parasiteEntamoeba were examined. The constituents inH. erectum alcoholic extracts and fractions separated by solvent-partitioning were analysed by high resolution LC –MS.Toxoplasma gondii growth inhibition assay was performed using GFP-labelledT. gondii strain PTG-GFP by measuring the fluorescence intensity. Anti-Toxoplasma drug pyrimethamine was used as a positive control.T. gondii-induced immune reaction was assessed by quantitative PCR and fluorescence microscopy, using co-culture of PTG-GFP and monocyte-macrophage cell line Raw264. The inhibitory effect on the encystation ofEntamoeba invadens was measured by flow-cytometry, where paromomycin was used as a positive control.H. erectum methanol (MeOH) extract (50  µg/mL) and ethyl acetate (EtOAc) fraction (50 µg/mL) inhibited the growth ofT. gondii, while 50%MeOH extract and hydrophilic fractions were ineffective. Co-culture withT. gondii reduced the viability of macrophages, however macrophages were protected in the presence ofH. erectum MeOH extract or EtOAc fraction (above 10  µg/mL). The MeOH extract and EtOAc fraction also effectively suppressed the encystation ofE. invadens at 1  mg/mL. Hypericine, a major constituent in MeOH ext...
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research

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This article aims to describe the current seek medical status of chronic liver diseases and discuss a stage-based consulting medical model for chronic liver diseases in China mainland, which would contribute to make rational use of limited medical resources and help to address National Health China 2030 strategy initiated by the Chinese government. PMID: 31711913 [PubMed - as supplied by publisher]
Source: Annals of Hepatology - Category: Gastroenterology Tags: Ann Hepatol Source Type: research
In conclusion, all extracts and petroleum ether extracts of the two plants have great potential to be developed as a novel acaricidal for controlling eggs and nymphs ofH. dromedarii, respectively.
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
Authors: Hoseini SM, Ronaghi H, Zaheri BA, Moshrefi AH, Adibi MA Abstract Spirocerca lupi is an international nematode of Canine and wild carnivores. The generic clinical symptoms are regurgitation, vomiting, and pursiness. The parasite usually procreates nodular masses in the esophagus and pectoral aorta. On July 2014, a four-month male sandy fox was found accidentally killed on the road in Bafgh City, Southeast of Yazd, Yazd Province, Iran during necropsy, referring to esophagus, parasites were collected from esophagus and transmissive up to 70% of alcohol. Parasites were recognized as S. lupi. After clarificatio...
Source: Iranian Journal of Parasitology - Category: Parasitology Tags: Iran J Parasitol Source Type: research
Authors: Berger EA Abstract Keratitis is a sight-threatening inflammatory condition of the cornea that can be caused by both infectious and non-infectious agents. Physical or chemical trauma are typically related to non-infectious keratitis, which may then become secondarily infected or remain non-infected. Etiology of infectious keratitis is most often associated with bacteria; but viruses, fungi, and parasites are common causative pathogens as well. As a global concern, common risk factors include: systemic immunosuppression (secondary to malnutrition, alcoholism, diabetes, steroid use), previous corneal surgery ...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
(Natural News) Antrodia cinnamomea, also known as camphor fungus, is a parasitic fungus that thrives in the inner cavity of Cinnamomum kanehirae, a tree endemic to Taiwan. It is considered a medicinal mushroom and is used by Taiwanese people to treat liver disease, in particular, liver injury caused by excessive alcohol drinking. In a recent study, researchers from National...
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
baş CS, Açıcı M Abstract A 25-year-old male patient, who had terminal stage skin cancer (malignant melanocytic neoplasia) on his skin of arm was infected with white, round, worm parasites in the lesion area of the skin. Parasites were collected with a thin forceps in a petri dish, cleaned in warm physiological saline, fixed by 70% hot ethyl alcohol and clarified in lactophenol, respectively. All 14 female and 7 male nematodes, some of which had partial autolysis, were identified as Ascaridia galli, a chicken nematode. Due to the fact that it is a very rare case in the medical literature, we wish to repor...
Source: Turkish Society for Parasitology - Category: Parasitology Authors: Tags: Turkiye Parazitol Derg Source Type: research
The pyrogenic property being the first activity described, members of the interleukin-1 superfamily (IL-1α, IL-1β, IL-18, and the newest members: IL-33, IL-36, IL-37, and IL-38) are now known to be involved in several inflammatory diseases such as obesity, atherosclerosis, cancer, viral and parasite infections, and auto-inflammatory syndromes as well as liver diseases. Inflammation processes are keystones of chronic liver diseases, of which the etiology may be viral or toxic, as in alcoholic or non-alcoholic liver diseases. Inflammation is also at stake in acute liver failure involving massive necrosis, and in i...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Publication date: Available online 19 August 2019Source: Pathology - Research and PracticeAuthor(s): Hui Wang, Rani KanthanAbstractAmoebiasis, caused by the intestinal protozoan Entamoeba histolytica, though a relatively common parasitic disease in the tropical and subtropical regions, is uncommon in the developed countries. In these countries, as amoebic colitis shares similar clinical symptoms and endoscopic features with inflammatory bowel disease (IBD), these cases can be easily unrecognized and misdiagnosed.In this case report, we discuss the case of an adult patient with invasive intestinal amoebiasis, who was initia...
Source: Pathology Research and Practice - Category: Pathology Source Type: research
In conclusion; our results ring a bell inviting the development of a roadmap for liver research in Egypt targeting to put future policies to cover areas of weakness in liver research with an ultimate goal of tackling liver disease and its overwhelming socioeconomic burden in our developing country.
Source: Arab Journal of Gastroenterology - Category: Gastroenterology Source Type: research
Conclusions/SignificanceCompared with the classical clinical-epidemiological profile of PCM caused byP.brasiliensis, the results of this descriptive study did not show significant clinical or epidemiological differences that could be attributed to the speciesP.lutzii. Future studies may confirm or refute the existence of clinical differences between the two fungal species.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
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