Dot by dot: analyzing the glycome using lectin microarrays.

Dot by dot: analyzing the glycome using lectin microarrays. Curr Opin Chem Biol. 2013 Jul 12; Authors: Ribeiro JP, Mahal LK Abstract The glycome, that is, the cohort of carbohydrates within a cell or tissue, plays a key part in diverse biological interactions involved in health and disease. Glycans are structurally complex and notoriously difficult to analyze. Lectin microarrays, a sensitive and high-throughput method for glycomic profiling, provide a global view of the glycome. In recent work, this technology has been successfully applied to a wide range of studies, from identification of glycan-based stem cell markers to the detection of pathogens and early diagnosis of disease. This review focuses on advances in the field of lectin microarrays. PMID: 23856055 [PubMed - as supplied by publisher]
Source: Current Opinion in Chemical Biology - Category: Biochemistry Authors: Tags: Curr Opin Chem Biol Source Type: research

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CONCLUSIONS: Our observations suggest that Notch signaling may have relevant roles in glomus coccygeum function and biology. PMID: 32087094 [PubMed - as supplied by publisher]
Source: Neuroendocrinology Letters - Category: Endocrinology Tags: Neuro Endocrinol Lett Source Type: research
Publication date: Available online 21 February 2020Source: Stem Cell ResearchAuthor(s): Yalan Zhou, Chengcheng Ding, Shutao Xia, Yutong Jing, Shengru Mao, Jian Liu, Juan Chen, Hon fai Chan, Shibo Tang, Jiansu ChenAbstractCrumbs homologue 1 (CRB1) mutations have been found in retinitis pigmentosa (RP) patients lead to severe retinal dystrophies. The human induced pluripotent stem (iPS) cell line CSUASOi003-A derived from peripheral blood mononuclear cells (PBMCs) of a patient carrying two heterozygous mutations (2249G>A p.G750D and c.2809G>A p.A937T) in CRB1 gene was generated by non-integrative reprogramming technolo...
Source: Stem Cell Research - Category: Stem Cells Source Type: research
Publication date: Available online 22 February 2020Source: Life SciencesAuthor(s): Maryam Ezzati, Bahman Yousefi, Kobra Velaei, Amin SafaAbstractBreast cancer is one of the well-known malignant tumors among women. In spite of attempts to classifying breast cancer according to its histological and molecular properties, it is almost considered as a dilemma in treatment. Nowadays, public and medical attentions have primary focused on foods with anti-cancer properties to alleviate the cancer problems. Flavonoid components such as Quercetin (Qu) as dietary substances with high attention of ordinary people might provide potentia...
Source: Life Sciences - Category: Biology Source Type: research
Publication date: Available online 22 February 2020Source: Life SciencesAuthor(s): Ning Zhou, Xiao-Bing Zhang, Cheng Chen, Xin-Yu Chen, Bo Kang, Jian-Qin He, Guo-Zhong Gong, Ying-Jie Wang, Yan-Wen ZhouAbstractAimsTo compare how OCT4A proteins interact with and regulate multiple OCT4A-octamer motifs (OMs) in different regions of the FOS gene expressed in somatic cancer cells versus pluripotent stem cells.Materials and methodsTwo FOS reporter gene systems harboring predicted OMs or their mutational counterparts were introduced into HeLa and NCCIT cells with varying OCT4A protein levels. The transcription of dsGFP reflecting ...
Source: Life Sciences - Category: Biology Source Type: research
Authors: Longoni A, Utomo L, van Hooijdonk IE, Bittermann GK, Vetter VC, Kruijt Spanjer EC, Ross J, Rosenberg AJ, Gawlitta D Abstract Dental pulp stem cells (DPSCs) are particularly promising for tissue engineering (TE) due to the ease of their isolation procedure, great expansion potential and capability to differentiate towards several cell types of the mesodermal, ectodermal and endodermal lineages. Although several studies hint that DPSCs exhibit potential for cartilage tissue formation, the chondrogenic potential of DPSCs has only been marginally explored. Thus, the aim of the present study was to closely inve...
Source: European Cells and Materials - Category: Cytology Tags: Eur Cell Mater Source Type: research
This study showed hub functional terms and gene participated in CC and then speculated that ACKR1 might be tumor suppressor for CC. PMID: 32083574 [PubMed - as supplied by publisher]
Source: Cancer Biomarkers - Category: Cancer & Oncology Tags: Cancer Biomark Source Type: research
CONCLUSION: This study reviewed SCT outcomes for pediatric AA with changes of transplant strategies over the last 25 years. The FFS and GFFS were higher in Flu-group than in Cy-group, especially in matched related transplantation. Graft failure including donor-type aplasia remains troublesome even with Flu-based conditioning. Further refinement of transplant strategies to ensure better quality-of-life should be pursued. PMID: 32080987 [PubMed - in process]
Source: Journal of Korean Medical Science - Category: Biomedical Science Tags: J Korean Med Sci Source Type: research
ConclusionsRisk and outcome of severe oral mucositis could be ameliorated by oral cryotherapy during the administration of cytotoxic agents for allogeneic HSCT patients receiving non-radiation myeloablative conditioning regimen, and a half-course schedule could acquire a comparable efficacy compared with the whole-course schedule.
Source: Supportive Care in Cancer - Category: Cancer & Oncology Source Type: research
Contributors : Katarina Szikszai ; Zdenek Krejcik ; Jiri Klema ; Nikoleta Loudova ; Andrea Mrhalkova ; Monika Belickova ; Jitka Vesela ; Monika Hruba ; Viktor Stranecky ; David Kundrat ; Jaroslav Cermak ; Anna Jonasova ; Michaela Dostalova MerkerovaSeries Type : Expression profiling by arrayOrganism : Homo sapiensMyelodysplastic syndromes (MDS) present a wide range of hematopoietic stem cell disorders with a tendency to evolve into acute leukemia. Long noncoding RNAs (lncRNAs) play multiple roles in hematopoiesis, contributing to the pathogenesis of hematologic malignancies and representing a new class of biomarkers and th...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research
This study investigated the control of 5-LO expression and leukotriene biosynthesis following the maturation of human monocytic cells. MonoMac-1 (MM1) and THP-1 cells were incubated for up to 72h with or without LPS and TGF-β. LPS, but not TGF-β, increased CD14 expression in both MM1 and THP-1 cells. Incubation with LPS (100 ng/ml) and TGF-β (1 ng/ml) synergistically increased the capacity of MM1 cells to produce 5-LO products from undetectable levels to 40±5 pmol/106 cells. 5-LO product biosynthesis in THP-1 cells increased 25-fold. A synergistic effect of LPS and TGF-β was measured with increas...
Source: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) - Category: Lipidology Source Type: research
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