Combined of salicylic acid and Pseudomonas fluorescens CHA0 on the expression of PR1 gene and control of Meloidogyne javanica in tomato

This study examined inducing resistance to tomato against Meloidogyne javanica, as well as time-dependent changes in the expression of PR1 gene, after applying salicylic acid (SA) and Pseudomonas fluorescens CHA0 (Pf) in the leaf tissue of healthy and infected tomatoes. Combined application of SA and Pf improved the plant growth and reduced root-galling, the number of eggs and egg-masses and reproduction factor. The results of the experiment of gene expression revealed that in all of the treatments, the expression of PR1 gene changed at different time points. The expression of PR1 gene in infected plants, 24 h after inoculation with nematode, was 98% higher than that of the control plants and 48 h after nematode inoculation, was about 58% less than that of control plants. The maximum expression of PR1 gene in nematode infected plants treated with Pf + SA was observed eight days after soil drench application of Pf that was 48 h after treatment with SA and 24 h after nematode inoculation and nine days after application of Pf, downregulation of the PR1 gene occurred. This study revealed that SA and Pf improved the plant growth indices and reduced the infection of Meloidogyne javanica in tomato plants and also it was observed that the expression of PR1 gene was significantly reduced in treatments with Pf and SA, as compared to their combination.Graphical abstract
Source: Biological Control - Category: Biology Source Type: research