Long noncoding RNA KCNQ1OT1 induces pyroptosis in diabetic corneal endothelial keratopathy.

Long noncoding RNA KCNQ1OT1 induces pyroptosis in diabetic corneal endothelial keratopathy. Am J Physiol Cell Physiol. 2019 Nov 06;: Authors: Zhang Y, Song Z, Li X, Xu S, Zhou S, Jin X, Zhang H Abstract Diabetic corneal endothelial keratopathy is an intractable ocular complication which seriously threaten vision. It has been suggested that diabetes is associated with pyroptosis, a type of inflammatory programmed cell death. Long noncoding RNA KCNQ1OT1 is involved in pathophysiological mechanisms of diabetic complications, including diabetic cardiomyopathy and diabetic retinopathy. The aim of this study was to investigate the mechanisms of KCNQ1OT1 in diabetic corneal endothelial keratopathy. Here, we reveal that KCNQ1OT1 and pyroptosis can be triggered in diabetic human and rats corneal endothelium, along with the high glucose-treated corneal endothelial cells. However, miR-214 expression was substantially decreased in vivo and in experiments with cultured cells. LDH assay verified pyroptosis in high glucose-treated cells. Bioinformatics prediction and luciferase assays showed that KCNQ1OT1 may bind miR-214 to regulate the expression of caspase-1. We further transfectec miR-214 mimic and inhibitor into the high glucose-treated corneal endothelial cells. The results showed that upregulation of miR-214 attenuated pyroptosis; conversely, knockdown of miR-214 promoted it. In addition, KCNQ1OT1 knockdown by a small interfering RNA decreas...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Tags: Am J Physiol Cell Physiol Source Type: research