Proteomic analysis reveals a role for RSK in p120-catenin phosphorylation and melanoma cell-cell adhesion.

In this study, we used a proximity-based labeling approach to identify RSK proximity partners in cells. We identified many potential RSK interacting proteins, including p120ctn (p120-catenin), which is an essential component of adherens junction (AJ). We found that RSK phosphorylates p120ctn on Ser320, which appears to be constitutively phosphorylated in melanoma cells. We also found that RSK inhibition increases melanoma cell-cell adhesion, suggesting that constitutive RAS/MAPK signaling negatively regulates AJ integrity. Together, our results indicate that RSK plays an important role in the regulation of melanoma cell-cell adhesion. PMID: 31678930 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/31678930?dopt=Abstract

Source: Molecular and Cellular Proteomics : MCP - November 1, 2019 Category: Molecular Biology Authors: Meant A, Gao B, Lavoie G, Nourreddine S, Jung F, Aubert L, Tcherkezian J, Gingras AC, Roux PP Tags: Mol Cell Proteomics Source Type: research