FAST-Mag protocol with or without mild hypothermia (35̊C) does not improve outcome after permanent MCAO in rats.

FAST-Mag protocol with or without mild hypothermia (35̊C) does not improve outcome after permanent MCAO in rats.
Magnes Res. 2013 Jun 28;
Authors: Meloni BP, Cross JL, Brookes LM, Clark VW, Campbell K, Knuckey NW
Abstract
The current study assessed the neuroprotective efficacy of magnesium using a FAST-Mag trial treatment protocol alone, and in combination with mild hypothermia, in Sprague Dawley rats subjected to permanent, middle cerebral artery occlusion (MCAO). Treatment with magnesium (MgSO4.7H2O) consisted of an intravenous loading dose (LD: 360 μmol/kg) and a 24 hour infusion (120 μmol/kg/h), while mild hypothermia at 35̊C was maintained for 24 hours. Treatment groups consisted of animals receiving: i) saline; ii) magnesium LD/infusion at 1.5 h/2.5 h post-MCAO; iii) magnesium LD/infusion at 1.5 h/2.5 h post-MCAO and hypothermia commencing at 2.5 h post-MCAO; iv) magnesium LD and hypothermia at 1.5 h and magnesium infusion at 2.5 h post-MCAO; v) hypothermia commencing at 1.5 h post-MCAO and magnesium LD/infusion at 2.5 h post-MCAO; and vi/vii) hypothermia commencing at 1.5 h or 2.5 h post-MCAO. No treatment significantly reduced infarct volumes or improved adhesive tape removal time when measured 48 hours after MCAO. These findings indicate that FAST-Mag treatment alone or with mild hypothermia may not provide benefit after ischemic stroke, associated with permanent cerebral artery occlusion.
PMID: 23816810 [PubMe...

Source: Magnesium Research - June 28, 2013 Category: Biochemistry Authors: Meloni BP, Cross JL, Brookes LM, Clark VW, Campbell K, Knuckey NW Tags: Magnes Res Source Type: research