Surface modification of decellularized trachea matrix with collagen and laser micropore technique to promote cartilage regeneration.

Surface modification of decellularized trachea matrix with collagen and laser micropore technique to promote cartilage regeneration. Am J Transl Res. 2019;11(9):5390-5403 Authors: Xu Y, Li Y, Liu Y, Li H, Jia Z, Tang Y, Jiang G, Zhang X, Duan L Abstract The repair of long-segment tracheal defects remains a significant clinical challenge, to which, optimal biologically functioning tracheal alternatives may serve as a solution. Tissue-engineered trachea, regenerated from a decellularized trachea matrix using the laser micropore technique (LDTM), demonstrates the possibility of developing optimal tracheal substitutes, which retain the original tubular shape and adequate cartilage regeneration ability of trachea. However, the strict requirement with respect to the implantation cell density restricts the clinical translation of the LDTM, which has a low cell adherence rate. To overcome this problem, we propose a novel strategy involving collagen to modify the LDTM surface in order to enhance cell retention efficiency and promote homogeneous tracheal cartilage regeneration. The current results show that the modified LDTM significantly improves cell-seeding efficiency; moreover, it achieved stable cell retention and homogenous cell distribution. Additionally, at a relatively low implantation cell density (5.0 × 107 cells/mL, which is one-fourth of the cell-seeding density used in our previous study), homogeneous tubular cartilage was regen...
Source: American Journal of Translational Research - Category: Research Tags: Am J Transl Res Source Type: research